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LC/MS Analysis of Vaccinium Anthocyanidins on Ascentis® Express C18 at Elevated Temperature and Flow

LC/MS Analysis of Vaccinium Anthocyanidins on Ascentis® Express C18 at Elevated Temperature and Flow application for HPLC

Materiales

columna analítica

Referencia del producto
Descripción
Precios

Columna para HPLC Ascentis® Express C18, 2,7 μm

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

patrón

Referencia del producto
Descripción
Precios

Pelargonidin chloride

Delphinidin chloride

analytical standard

Peonidin 3-O-glucoside chloride

analytical standard

Cyanidin chloride

≥95% (HPLC)

Malvidin chloride

analytical standard

CONDITIONS

column

Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)

mobile phase

[A] 0.1% formic acid in water; [B] 0.1% formic acid in 75:25 (v/v) acetonitrile:water; (78:22, A:B)

flow rate

0.6 mL/min

pressure

5440 psi (375 bar)

column temp.

35 °C

detector

ESI(+), TOF

injection

1 μL

sample

20 μg/mL in 0.1% formic acid

Descripción

Nota de análisis

Fruits of the genus Vaccinium are popular sources for anthocyanidin antioxidants. This genus includes the commonly known blueberry, huckleberry, and bilberry.

Información legal

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany