822854

Columna de HPLC de exclusión por tamaño (tipo SW) TSKgel^®

phase diol, L × I.D. 30 m × 7.8 mm, 4 μm particle size

• Sinónimos: TSKgel^® Size Exclusion (SW-Type) HPLC Column
• Código UNSPSC: 41115711

Propiedades

• Materiales: stainless steel
• Agency: suitable for USP L20; suitable for USP L59
• Línea del producto: TSKgel^®
• fabricante / nombre comercial: Tosoh SuperSW mAb HR, 22854
• Parámetros: ≤0.5 M salt concentration; 0-100% aqueous soluble organic solvents; 1 mL/min flow rate; 10-30 °C temperature; 120 bar max. pressure
• técnicas: HPLC: suitable
• L × D.I.: 30 m × 7.8 mm
• matriz: silica particle platform
• grupo activo de la matriz: diol phase
• tamaño de partícula: 4 μm
• tamaño de poro: 250 Å pore size
• pH operativo: 2.5-7.5
• técnica de separación: size exclusion (SEC)

Descripción

Descripción general

The TSKgel SuperSW and UltraSW mAb columns consist of three specialized columns designed for the separation and analysis of monoclonal antibodies (mAb). While mAbs can be analyzed using many different modes of HPLC, size exclusion is best to determine the level of aggregation, dimer formation, and fragmentation, making it the best method for heterogeneity studies. TSKgel SuperSW mAb HR columns are optimized for high resolution analysis of mAb monomer and dimer; these 30 cm x 7.8 mm ID slica-based stainless steel columns offer reduced lot-to-lot variation, long column life, reduction of unspecified adsorption, and superior recovery of aggregates. The TSKgel SuperSW mAb columns utilize a unique pore-controlled technology, which produces a shallow calibration curve in the molecular weight region of a typical monoclonal antibody. The calibration curve for the TSKgel SuperSW mAb HR column is similar to that of the industry-leading TSKgel G3000SWxl column and has a shallower slope than the TSKgel UltraSW Aggregate column around the molecular weight range of gamma-globulin. This shallow calibration curve produces high resolution separations.

Aplicación

TSKgel^® superSW mAb HR HPLC column may be used in the separation of monoclonal antibodies using size exclusion chromatography (SEC).

Forma física

Shipped in 0.05% NaN3 and 0.1 M Na2SO4 in 0.1 M phosphate buffer, pH 6.7.

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Información legal

TSKgel is a registered trademark of Tosoh Corporation