814014

TSKgel^® Ether-5PW (10 µm) HPLC Columns

L x I.D. 7.5 cm × 8 mm, ether phase

• Código UNSPSC: 41115711

Propiedades

• product name: TSKgel^® Ether-5PW HPLC Column, phase ether, L × I.D. 7.5 cm × 8 mm, 10 μm particle size
• Materiales: glass
• Línea del producto: TSKgel^®
• fabricante / nombre comercial: Tosoh 14014
• Parámetros: ≤4 M salt concentration; ≤50% organic solvent; 1.2 mL/min flow rate; 10-50 °C temperature; 20 bar max. pressure
• técnicas: HPLC: suitable
• L × D.I.: 7.5 cm × 8 mm
• matriz: hydroxylated methacrylic polymer
• grupo activo de la matriz: ether phase
• tamaño de partícula: 10 μm
• pH operativo: 2-12
• técnica de separación: hydrophobic interaction (HIC)

Descripción

Descripción general

Both hydrophobic interaction chromatography (HIC) and reversed-phase liquid chromatography separate on the basis of protein hydrophobicity and allow selective binding and desorption of proteins. However, HIC operates at significantly lower binding energy and uses aqueous mobile phases. These characteristics provide a gentle technique that is less likely to disturb protein conformation. As a result, HIC generally provides better activity recovery.
Three resin-based TSKgel HIC columns provide a range of hydrophobicities for chromatographic optimization. TSKgel Phenyl-5PW columns are most hydrophobic, followed by Ether-5PW, while the surface of Butyl-NPR columns is most polar or least hydrophobic. Ammonium sulfate concentration can be minimized by using a more hydrophobic packing such as Phenyl-5PW. We recommend the more polar Ether-5PW column for purifying more hydrophobic proteins. Ether-5PW and Phenyl-5PW packings are based on TSKgel G5000PW resin - 10 micron particles with 1000  pores. Butyl-NPR packing is prepared from 2.5 micron nonporous particles, allowing for rapid analyses and semi-micropreparative applications. All three column types can be cleaned with 0.2 M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.

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Información legal

TSKgel is a registered trademark of Tosoh Corporation