ZRB2140

Anti-TYK2 Antibody, clone 2H22 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: Non-receptor tyrosine-protein kinase TYK2
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 2H22, recombinant monoclonal
• descripción: 2H22 Clone
• Línea del producto: ZooMAb^® learn more
• formulario: lyophilized
• mol peso: calculated mol wt 133.65 kDa; observed mol wt ~135 kDa
• purificado por: using Protein A
• reactividad de especies: human, monkey
• reactividad de especies (predicha por homología): porcine, bovine
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG
• secuencia del epítopo: C-terminal half
• Nº de acceso Protein ID: NP_003322
• Nº de acceso UniProt: P29597
• categoría alternativa más sostenible: Aligned,
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... Tyk2(7297)

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone 2H22 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Non-receptor tyrosine-protein kinase TYK2. It targets an epitope within 23 amino acids from the C-terminal half.

Inmunógeno

KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal half of human Non-receptor tyrosine-protein kinase TYK2.

Aplicación

Quality Control Testing

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TYK2 in Raji cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TYK2 in Jurkat and COS-7 cell lysates.

Affinity Binding Assay: A representative lot of this antibody bound TYK2 peptide with a KD of 8.7 x 10-9 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TYK2 in Human fallopian tube tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

Non-receptor tyrosine-protein kinase TYK2 (UniProt: P29597; also known as Tyk2) is encoded by the Tyk2 gene (Gene ID: 7297) in human.TYK2 is a ubiquitously expressed member of the Janus kinase (JAK) family that is associated with receptors of type I interferon, interleukins 6, 10, 12, and 23 and plays a key role in the signal transduction of these cytokines. It is important in both innate and adaptive immune cells and is shown to be an essential component of maintaining normal immune responses. TYK2 is also involved in various other processes, including cell growth, development, and cell migration. It is reported to associate with growth factor receptors and activates STAT family of transcription factors. STAT proteins that homo- or heterodimerize following their phosphorylation translocate to the nucleus and regulate cytokine responsive genes. Its protein kinase 1 domain (pseudokinase domain), located in amino acids 589-875, mediates autoinhibition of the TYK2 kinase domain. Phosphorylation of tyrosine 1054 and 1055 induces kinase domain (aa 897-1176) activation. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wu, P., et al. (2020). Front. Pediatr. 8; 253; Lupardus, PJ., et al. (2014). Proc. Natl. Acad. Sci. USA. 111 (22) 8025-8030; Yeh, TC., et al. (2000). Proc. Natl. Acad. Sci. USA. 97(16); 8991-8996; Rani, MR., et al. (1999). J. Biol. Chem. 274(45); 32507-32511).

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitución

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable