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Merck

V5265

Sigma-Aldrich

Crystal Violet Solution

1%, aqueous solution

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About This Item

Fórmula empírica (notación de Hill):
C25H30ClN3
Número de CAS:
Peso molecular:
407.98
Beilstein:
3580948
Número MDL:
Código UNSPSC:
12171500
ID de la sustancia en PubChem:
NACRES:
NA.47

Formulario

aqueous solution

Nivel de calidad

concentración

1%

técnicas

microbe id | staining: suitable

color

deep violet, purple

Amáx.

0.36-0.44 at 589-594 nm

aplicaciones

diagnostic assay manufacturing
hematology
histology

temp. de almacenamiento

room temp

cadena SMILES

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

Clave InChI

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

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Descripción general

Crystal violet, also known as gentian violet or methyl violet, is the N-hexamethylated derivative of pararosaniline. It is a basic, cationic, acidotropic protein dye used in histological and bacteriological staining. It is well-known for its antibacterial and antifungal properties.

It stains the fatty portions of sebaceous sweat a deep purple color. Crystal violet can also be used to enhance bloody fingerprints. This dye is harmful if inhaled, swallowed or absorbed through skin, contact may cause cancer, severe eye irritation in human beings.

Aplicación

  • Crystal violet is commonly used in Gram staining for the classification of bacteria.
  • It has also been used to detect bacterial adherence to biomedical polymers and to stain DNA in mammalian tissues in Giemsa staining.
  • It has been successfully used to develop a counterion-staining method to detect DNA in agarose gel electrophoresis.
  • Its antimicrobial properties have facilitated its use in the treatment of oral candidiasis, skin infections, and methicillin-resistant Staphylococcus aureus.
  • It has been used as a stain in cell proliferation assays, migration assays, and Boyden chamber assay.
Crystal violet solution for use in Brown-Hopps method for Gram-positive and Gram-negative bacterial staining.

Acciones bioquímicas o fisiológicas

The antimicrobial effects of crystal violet can be attributed to free radical generation and formation of unionized bacterial complexes with the dye. It has also been reported to inhibit protein synthesis and bacterial cell wall formation. It is especially effective against Gram-positive bacteria.

Principio

Crystal violet is the primary stain in Gram staining. It infiltrates the peptidoglycan-rich cell wall of Gram-positive bacteria, and forms entrapped complexes with Gram′s iodine that retain the purple color post-decolorization. Thus, Gram-positive bacteria can be differentiated from Gram-negative bacteria whose peptidoglycan layer is considerably thinner.

Pictogramas

Health hazardExclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Listados normativos

Los listados normativos se proporcionan para los productos químicos principalmente. Para los productos no químicos sólo se puede proporcionar información limitada. Si no hay ninguna entrada, significa que ninguno de los componentes está en la lista. Es obligación del usuario garantizar el uso seguro y legal del producto.

EU REACH Annex XVII (Restriction List)

CAS No.

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Certificados de análisis (COA)

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