C0775

Crystal Violet

Dye content, ≥90%, certified by the Biological Stain Commission, powder

• Sinónimos: Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B
• Fórmula lineal: C₂₅H₃₀N₃Cl
• Número de CAS: 548-62-9
• Peso molecular: 407.98
• Número de índice de color: 42555
• Beilstein: 3580948
• Número CE: 208-953-6
• Número MDL: MFCD00011750
• Código UNSPSC: 12171500
• ID de la sustancia en PubChem: 57653980
• NACRES: NA.47

Propiedades

• product name: Crystal Violet, certified by the Biological Stain Commission
• grado: certified by the Biological Stain Commission
• Nivel de calidad: 200
• formulario: powder
• composición: Dye content, ≥90%
• color: green to very dark green
• pH: 2.5 — 3.5 (20 °C)
• mp: 205 °C (dec.) (lit.)
• solubilidad: H₂O: 1 mg/mL
• densidad: 1.19 g/cm³ at 20 °C
• espectro de actividad antibiótica: fungi
• aplicaciones: diagnostic assay manufacturing; hematology; histology
• Modo de acción: cell membrane | interferes; enzyme | inhibits
• temp. de almacenamiento: room temp
• cadena SMILES: [Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C
• InChI: 1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1
• Clave InChI: ZXJXZNDDNMQXFV-UHFFFAOYSA-M

Descripción

Aplicación

Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria.
It has been used to check cell viability. It has also been used for the staining of cells to study cell migration and invasion.

Acciones bioquímicas o fisiológicas

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal Violet also helps in colorimetric measurement of cell viability.

Idoneidad

Certified for use with the Gram stain for bacteria, Flemming triple stain with iodine for chromosomes, Lieb′s amyloid stain and Newton′s crystal violet-iodine technique for chromatin and nucleoli.

Información de seguridad

• Pictogramas: GHS08,GHS05,GHS07,GHS09
• Palabra de señalización: Danger
• Frases de peligro: H302,H318,H351,H410
• Consejos de prudencia: P202 - P273 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Clasificaciones de peligro: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable