UC9601
UltraClear™ Sequencing Reaction Clean-Up Kit
pkg of 1 plates, sufficient for 1 96-well plate purifications
Sinónimos:
Sequencing Clean-Up Kit, UltraClear Reaction Kit
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About This Item
Código UNSPSC:
41105600
NACRES:
NA.52
Productos recomendados
uso
sufficient for 1 96-well plate purifications
Nivel de calidad
envase
pkg of 1 plates
temp. de almacenamiento
15-25°C
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Descripción general
The UltraClear Sequencing Reaction Clean-Up Plates offer a rapid and simple method for the clean-up of DNA sequencing reactions in a 96-well format. The plates use ultrafiltration membranes to separate low molecular weight contaminants, such as unincorporated dye terminators, dNTPs, and residual salts from the sequencing reaction products.
Following thermocycling, the sequencing reactions are diluted with Sequencing Solution. This mixture is then filtered through the UltraClear plate by centrifugation. The sequencing reaction products are retained on the surface of the ultrafiltration membrane while the lower molecular weight contaminants pass through the membrane and are collected as waste. The purified sequencing products are then resuspended in Sequencing Solution and are ready for injection onto capillary-based DNA analyzers.
Following thermocycling, the sequencing reactions are diluted with Sequencing Solution. This mixture is then filtered through the UltraClear plate by centrifugation. The sequencing reaction products are retained on the surface of the ultrafiltration membrane while the lower molecular weight contaminants pass through the membrane and are collected as waste. The purified sequencing products are then resuspended in Sequencing Solution and are ready for injection onto capillary-based DNA analyzers.
Características y beneficios
- Effective clean-up of ABI BigDye® chemistries
- Simple centrifugation-based procedure
- Convenient 96-well format
Otras notas
For additional information, please see www.sigma-aldrich.com/sequencing.
Información legal
BigDye is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
UltraClear is a trademark of Sigma-Aldrich Co. LLC
Código de clase de almacenamiento
10 - Combustible liquids
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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