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U8501

Sigma-Aldrich

Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast

Type X, lyophilized powder, ≥40 units/mg protein

Sinónimos:

UTP:α-D-glucose-1-phosphate uridylyltransferase

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About This Item

Número de CAS:
9026-22-6
Comisión internacional de enzimas:
2.7.7.9 (BRENDA, IUBMB)
Número CE:
232-811-2
Número MDL:
MFCD00132561
Código UNSPSC:
12352204
NACRES:
NA.54

origen biológico

bakers yeast

Nivel de calidad

200

tpo

Type X

formulario

lyophilized powder

actividad específica

≥40 units/mg protein

composición

Protein, 30-60% modified Warburg-Christian

actividad extraña

UDP-glucose dehydrogenase and galactose-1-phosphate uridyltransferase ≤0.1%
inorganic pyrophosphatase ≤0.5%

temp. de almacenamiento

−20°C

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Descripción general

Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) is ubiquitous in plants, yeast, bacteria, and mammals. This enzyme is an octamer of eight identical sub-units.

Aplicación

Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast has been used:
  • in the synthesis of uridine-5′-diphosphoglucose (UDP-Glc)-13C9
  • to quantify Suc synthase (SUS) enzyme activity in rice
  • to study the role of hexokinase and glycogen synthase controls the flux in frog oocytes

Acciones bioquímicas o fisiológicas

Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) participates in catalyzing the synthesis of UDP-glucose. This enzyme requires divalent cations such as Mg2+, Ca2+, Mn2+, Ni2+ for its activity.

Definición de unidad

One unit will form 1.0 μmole of glucose 1-phosphate from uridine-5′-diphosphoglucose and inorganic pyrophosphate per min at pH 7.6 at 25 °C.

Forma física

Lyophilized, sulfate-free powder containing citrate buffer salt

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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D J Sutor et al.
Clinica chimica acta; international journal of clinical chemistry, 86(3), 329-332 (1978-06-15)
The concentration of pyrophosphate in urine has been determined using the enzyme uridine-5'-diphosphoglucose pyrophosphorylase. The technique is relatively quick and easy to perform. Reproducibility of results was good, and results from recovery experiments were excellent. The mean concentration of pyrophosphate
Himangi R Jayakar et al.
BMC microbiology, 11, 179-179 (2011-08-09)
A number of studies have revealed that Francisella tularensis (FT) suppresses innate immune responses such as chemokine/cytokine production and neutrophil recruitment in the lungs following pulmonary infection via an unidentified mechanism. The ability of FT to evade early innate immune
Leszek A Kleczkowski et al.
The Biochemical journal, 439(3), 375-379 (2011-10-14)
Plant pyrophosphorylases that are capable of producing UDP-sugars, key precursors for glycosylation reactions, include UDP-glucose pyrophosphorylases (A- and B-type), UDP-sugar pyrophosphorylase and UDP-N-acetylglucosamine pyrophosphorylase. Although not sharing significant homology at the amino acid sequence level, the proteins share a common
Yonglan Yu et al.
PloS one, 5(9), e12593-e12593 (2010-09-11)
The trehalose synthetic pathway is present in bacteria, fungi, plants and invertebrate animals, but is absent in vertebrates. This disaccharide mainly functions as a stress protectant against desiccation, heat, cold and oxidation. Genes involved in trehalose synthesis have been observed
Jesús Rodríguez-Díaz et al.
Journal of biotechnology, 154(4), 212-215 (2011-06-15)
UDP-sugars are widely used as substrates in the synthesis of oligosaccharides catalyzed by glycosyltransferases. In the present work a metabolic engineering strategy aimed to direct the carbon flux towards UDP-glucose and UDP-galactose biosynthesis was successfully applied in Lactobacillus casei. The
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