TRUE7

TrueGel3D Hydrogel Kits

SLO-DEXTRAN, allow cell recovery, CD cell-degradable crosslinker

• Código UNSPSC: 12352207
• NACRES: NA.71

Propiedades

• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C

Descripción

Descripción general

The gel is formed by stable thioether bonds between thiol groups on CD cell-degradable crosslinker and thio-reactive groups on SLO-DEXTRAN. The polymer, SLO-DEXTRAN is functionalized with thiol-reactive groups which react with crosslinker at slower rate than maleimides in FAST-DEXTRAN. SLO-Dextran does not have sites for cell attachment, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1UM) prior to the crosslinking step.

The CD cell-degradable crosslinker is composed of matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala), which allows cells to spread and migrate by secreting matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9).

TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.

Aplicación

TRUE7-1KT is used to generate hydrogels of different stiffness (0.15 to 5 KPa shear modulus) to mimic in-vivo conditions and to investigate the effect of mechanical stimuli on cell physiology and behavior. The SLO-DEXTRAN based hydrogels are used in microchannels and also in syringes for injection. These hydrogels are also employed to study cell migration of neutrophils, dendritic cells and lymphocytes.

Características y beneficios

• Slow gelling (takes 2.5 minutes to 6 minutes, depends on gel stiffness)
• Flexibility in modulating gel stiffness
• Contains MMP-cleavable crosslinker
• Allow cell recovery and post-culture analyses
• Cell inert hydrogel (if not customized with RGD peptide)

Componentes

TRUE7-1KT contains SLO-DEXTRAN solution in phosphate buffer and lyophilized CD cell-degradable crosslinker with reconstitution buffer and water.

Precaución

Avoid prolonged exposure of CD cell-degradable crosslinker to air.

Información de seguridad

• Pictogramas: GHS05
• Palabra de señalización: Warning
• Frases de peligro: H290,H315,H319
• Consejos de prudencia: P234 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Clasificaciones de peligro: Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
• Código de clase de almacenamiento: 8A - Combustible corrosive hazardous materials
• Clase de riesgo para el agua (WGK): WGK 3