T9378

Trypsin inhibitor

crude powder

• Sinónimos: Trypsin Inhibitor from lima beans
• Número de CAS: 9035-81-8
• Número CE: 232-906-9
• Número MDL: MFCD00082102
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• product name: Trypsin inhibitor from Phaseolus limensis (lima bean), Type II-L, crude powder
• origen biológico: Phaseolus limensis (lima bean)
• tpo: Type II-L
• formulario: crude powder
• mol peso: 9 kDa
• solubilidad: 0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL
• temp. de almacenamiento: 2-8°C

Descripción

Aplicación

Trypsin inhibitor from Phaseolus limensis (lima bean) has been used:

• in the inhibition of trypsin in human plasma
• in the termination of proteolytic digestion in rabbit skeletal muscles
• in the inhibition of salivary glands enzyme extract from Lygus lineolaris
• in the inhibition of proteolytic activity in Agave tequilana enzyme extract

Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type enzymes.

Acciones bioquímicas o fisiológicas

Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors. It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains.

Propiedades físicas

Monomer has an apparent molecular weight of approx. 9,000 Da but undergoes a concentration and pH-dependent dimerization.

Definición de unidad

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

Nota de preparación

Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified).

Nota de análisis

One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Otras notas

View more information on Trypsin Inhibitor.

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H317,H334
• Consejos de prudencia: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Clasificaciones de peligro: Resp. Sens. 1 - Skin Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)