10109878001
Roche
Trypsin Inhibitor
from chicken egg white
Sinónimos:
trypisin
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About This Item
Código UNSPSC:
12352207
Productos recomendados
origen biológico
chicken egg white
Nivel de calidad
formulario
powder
envase
pkg of 1 g
fabricante / nombre comercial
Roche
concentración
1 mg/mL
técnicas
cell culture | mammalian: suitable
pH óptimo
3.8-4.5
solubilidad
water: soluble
temp. de almacenamiento
2-8°C
Descripción general
Egg white trypsin inhibitor is an inhibitor for trypsin, plasmin and plasma kallikrein activity in serum-free cell-culture media. It does not inhibit metallo-, cysteine, and aspartic proteases or tissue kallikrein (serine protease).
Aplicación
Chicken egg Trypsin Inhibitor is used for the termination of tissue disaggregation and for subcultivation procedures.
Trypsin Inhibitor has been used:
- as a component of lysis buffer to treat Tsc2fl/fl (control) and Tsc2fl/flLyz2-Cre (knockout [KO]) bone marrow-derived macrophages (BMDMs) before immunoblotting
- to inactivate trypsin post detachment of the stem cells in ventricular zone cell culture
- to neutralize trypsin post dissociation and trypsinization of brain tissues
- termination of tissue disaggregation and for subcultivation procedures
Nota de preparación
Working concentration: 0
Working solution: Solvent is recommended in distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
Stable for at least 6 months in distilled water or 1 mM HCI when stored frozen in aliquots. Avoid repeated freeze-thaw cycles.
Working solution: Solvent is recommended in distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
Stable for at least 6 months in distilled water or 1 mM HCI when stored frozen in aliquots. Avoid repeated freeze-thaw cycles.
Reconstitución
Both are soluble in water. Recommended stock solution: 1 mg/ml. Store frozen in aliquots at -15 to -25 °C. Stable for at least 6 months.
Almacenamiento y estabilidad
Store at 2 to 8 °C. (Store dry!)
Otras notas
For life science research only. Not for use in diagnostic procedures.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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