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Merck

T8690

Sigma-Aldrich

Thioredoxin human

≥90% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal histidine tagged), essentially salt-free, lyophilized powder, ≥5 U/mg

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About This Item

Número de CAS:
Número MDL:
Código UNSPSC:
12352200
NACRES:
NA.32

origen biológico

human

Nivel de calidad

recombinante

expressed in E. coli (N-terminal histidine tagged)

Análisis

≥90% (SDS-PAGE)

formulario

essentially salt-free, lyophilized powder

actividad específica

≥5 U/mg

mol peso

14 kDa

técnicas

activity assay: suitable

solubilidad

water: soluble 0.50—0.60 mg/mL, clear to slightly hazy, colorless to faint yellow or tan

idoneidad

suitable for molecular biology

Nº de acceso UniProt

aplicaciones

cell analysis

temp. de almacenamiento

−20°C

Información sobre el gen

human ... TXN(7295)

Descripción general

Cloned from Jurkat cell cDNA. Identical to the ADF/Trx (GenBank® Accession Number X77584) and the thioredoxin from human placenta (GenBank® Accession Number AF085844).
Thioredoxin (TXN) is a 12 kDa ubiquitous protein. The gene is located on human chromosome 9q31.3. TXN is mainly located in the cytoplasm.Thioredoxin contains a redox-active dithiol in the active site. Itexists in several forms, the most prevalent ones being the cytosolic (TRX1) andmitochondrial (TRX2) forms.

Aplicación

Thioredoxin human can be used for in vitro thioredoxin assay. The product can also be used for assessing Thioredoxin reductase (TRXR) enzyme activity.
Thioredoxin human has been used:
  • in mitogen-activated protein kinase kinase 4 (MKK4) in vitro kinase assay
  • to study its role in transcription regulation, RNA processing and nuclear pore function in human cells
  • in proteomics identification of Trx1 reduction target proteins from the hearts of cardiac specific transgenic (Tg)-Trx1 mice

Acciones bioquímicas o fisiológicas

Human Thioredoxin is a recombinant, N-terminal histidine tagged protein. The product induces proliferation in lymphoid cells, fibroblasts as well as in numerous human solid tumor cell lines.The product also stimulates growth in normal as well as leukemic B cell, modifies the transcriptional activity of AP-1and inhibits the apoptosis signal-regulating kinase 1 (ASK1).Thioredoxin (TRX) plays a role in protecting the cells fromelectrophiles, a cytotoxic agent.
Thioredoxin (TXN) has disulfide-reducing activity. It acts as a cytokine to reduce inflammation. TXN functions as a scavenger for reactive oxygen intermediates (ROIs), which are produced in various oxidative stress. Thioredoxin induces cellular proliferation to decrease oxidative stress. TXN is a prognostic biomarker for various cancers, such as lung, pancreatic and hepatocellular carcinoma. It contributes to protein reduction, tissue development and redox regulation.
Mediates the reduction of disulfide bonds in proteins.

Definición de unidad

One unit will cause a ΔA650 of 1.0 in 1 min at 25 °C in the insulin reduction assay.

Información legal

GenBank is a registered trademark of United States Department of Health and Human Services

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Molecular and Cellular Proteomics, 8(7), 1674-1687 (2009)
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PloS one, 8(8), e71525-e71525 (2013-08-13)
Thioredoxin reductase-1 (TRXR-1) is the sole selenoprotein in C. elegans, and selenite is a substrate for thioredoxin reductase, so TRXR-1 may play a role in metabolism of selenium (Se) to toxic forms. To study the role of TRXR in Se
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Thioredoxin activates MKK4-NFkappaB pathway in a redox dependent manner to control manganese superoxide dismutase gene expression in endothelial cells
Kundumani-Sridharan V, et al.
The Journal of Biological Chemistry, jbc-M115 (2015)

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