T111030

DMT-dT Phosphoramidite

configured for ABI

• Sinónimos: 5′-O-(4,4′-dimethoxytrityl)-2′-deoxythymidine-3′-O-[O-(2-cyanoethyl)-N,N′-diisopropylphosphoramidite]
• Fórmula empírica (notación de Hill): C₄₀H₄₉N₄O₈P
• Número de CAS: 98796-51-1
• Peso molecular: 744.81
• Número MDL: MFCD00055063
• Código UNSPSC: 41116107
• ID de la sustancia en PubChem: 329826644
• NACRES: NA.51

Propiedades

• origen biológico: non-animal source (no BSE/TSE risk)
• Nivel de calidad: 200
• tpo: for DNA synthesis
• Línea del producto: Proligo Reagents
• Ensayo: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• Formulario: powder or granules
• técnicas: oligo synthesis: suitable
• impurezas: ≤0.3 wt. % water content (Karl Fischer)
• color: white to off-white
• λ: conforms (UV/VIS Identity)
• compatibilidad: configured for ABI
• perfil del nucleósido: base: deoxythymidine; base protecting group: none; 2' protecting group: none; 5' protecting group: DMT; deprotection: fast/standard
• temp. de almacenamiento: 2-8°C
• cadena SMILES: COc1ccc(cc1)C(OC[C@H]2O[C@H](C[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)N3C=C(C)C(=O)NC3=O)(c4ccccc4)c5ccc(OC)cc5
• InChI: 1S/C40H49N4O8P/c1-27(2)44(28(3)4)53(50-23-11-22-41)52-35-24-37(43-25-29(5)38(45)42-39(43)46)51-36(35)26-49-40(30-12-9-8-10-13-30,31-14-18-33(47-6)19-15-31)32-16-20-34(48-7)21-17-32/h8-10,12-21,25,27-28,35-37H,11,23-24,26H2,1-7H3,(H,42,45,46)/t35-,36+,37+,53?/m0/s1
• Clave InChI: UNOTXUFIWPRZJX-CEXSRUIHSA-N

Descripción

Descripción general

Proligo′s DNA phosphoramidites lead to high-yield and high-quality oligonucleotides due to their high coupling efficiency. The deprotection step of automated oligonucleotide synthesis is integral to synthesis time and final product quality. Exocyclic amine functions are protected by a benzoyl group (dA(bz) and dC(bz)) or isobutyryl group (dG(ib)). Recommended cleavage and deprotection conditions are 8 hours at 55 °C or 24 hours at room temperature using concentrated ammonia solution, for standard base-protected oligonucleotides. DMT-dT Phosphoramidite belongs to ABI^™ synthesizers.

Información legal

ABI is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

Información de seguridad

• Código de clase de almacenamiento: 13 - Non Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable