SRP6269

MMP-1 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Sinónimos: CLG, CLGN
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• origen biológico: human
• recombinante: expressed in HEK 293 cells
• etiqueta: 6-His tagged (N-terminus)
• Análisis: ≥95% (SDS-PAGE)
• formulario: lyophilized powder
• mol peso: calculated mol wt 52.7 kDa; observed mol wt 52-55 kDa (DTT-reduced. Phe 20 is the predicted N-terminus.)
• envase: pkg of 10 and 50 μg
• impurezas: <1 EU/μg endotoxin (LAL test)
• Nº de acceso UniProt: P03956
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... MMP-1(4312)

Descripción

Descripción general

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive pro-proteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Thrombospondins, intervertebral disc proteins, regulate the effective levels of matrix metalloproteinases (MMPs) 2 and 9, which are key effectors of ECM remodeling. Matrix metalloproteinase-1 (MMP-1) also known as interstitial collagenase and fibroblast collagenase. MMP1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. MMP-1 breaks down the interstitial collagens, types I, II, and III. MMP1 can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin, myelin basic protein, L Selectin, proTNF, IL1β, IGFBP3, IGFBP5, pro MMP2 and pro MMP9.

Forma física

Lyophilized from 0.22 μm filtered solution in MES, NaCl, CaCl2 and Brij­35. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable