M9445

Matrix Metalloproteinase-2 from mouse

recombinant, expressed in NSO cells, >95% (SDS-PAGE), buffered aqueous glycerol solution

• Sinónimos: 72 kD Gelatinase, 72 kD Type IV Collagenase, Gelatinase A, MMP-2
• Número MDL: MFCD01324322
• Código UNSPSC: 12352202
• NACRES: NA.32

Propiedades

• recombinante: expressed in NSO cells
• Nivel de calidad: 200
• Análisis: >95% (SDS-PAGE)
• formulario: buffered aqueous glycerol solution
• mol peso: apparent mol wt ~72 kDa
• Nº de acceso UniProt: P33434; P33436
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: mouse ... Mmp2(17390) ; rat ... Mmp2(81686)

Descripción

Descripción general

Matrix Metalloproteinase-2 (MMP-2) also known as gelatinase or type IV collagenase is a 72kDa protein. MMP-2 is a member of matrix metalloproteinase (MMP) family of enzymes. Basic structure of MMP2 contains signal peptide domain that targets the enzyme for secretion, the pro-peptide domain, which is removed when the enzyme is activated and the catalytic site containing gelatin-binding domain.

Especificidad

The amino acid sequences 1-662 of the proenzymes of MMP-2 are identical between mouse and rat.

Aplicación

Matrix metalloproteinase-2 (MMP2) human has been used as a standard in zymography to measure proteolytic activity of MMP-2.

Acciones bioquímicas o fisiológicas

Matrix Metalloproteinase-2 (MMP-2) cleaves gelatin, type IV, V, VII, X, and XI collagens, fibronectin, elastin, laminin, proteoglycans and a range of non extracellular matrix (ECM ) components. MMP-2 cleaves native type I collagen to N-terminal ¾ and C-terminal ¼ fragments identical to those generated by interstitial collagenases. MMP2 and MMP9 play an essential role in matrix degradation and they are implicated in the maintenance of neovascularization. In mice, deletion or inhibition of MMP2 protects against myocardial rupture.

MMP-2 degrades general matrix components and may have a role in processes such as host defense, cell proliferation, and protein turnover as well as tissue remodeling.

Forma física

Supplied as a 0.2 μm filtered solution of 25 mM Tris, pH 7.5, 5 mM calcium chloride, 75 mM sodium chloride, 0.025% Brij^® 35 and 50% glycerol.

Nota de análisis

The biological activity is measured by its ability to cleave a fluorogenic peptide sustrate.

Información legal

Brij is a registered trademark of Croda International PLC

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)