SRP3085

Interleukin-2, human

Animal-component free, recombinant, expressed in E. coli, suitable for cell culture

• Sinónimos: Aldesleukin, IL-2, T-cell growth factor (TCGF)
• Código UNSPSC: 12352202
• NACRES: NA.32

Propiedades

• origen biológico: human
• Nivel de calidad: 300
• recombinante: expressed in E. coli
• Ensayo: ≥95% (HPLC)
• Formulario: (Lyophilized with no additives from a 0.2µ filtered solution)
• potencia: ≤0.5 ng/mL ED₅₀
• mol peso: 15.5 kDa
• envase: pkg of 50 μg
• técnicas: cell culture | mammalian: suitable
• impurezas: ≤1.00 EU/μg endotoxin, tested
• Nº de acceso UniProt: P60568
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... IL2(3558)

Descripción

Descripción general

IL-2 (interleukin 2) is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is a type I cytokine. The gene is mapped to human chromosome 4q27. Recombinant human IL-2 is a 15.5kDa protein, containing 134 amino acid residues including one intrachain disulfide bond.

Aplicación

• IL-2 (interleukin 2) human has been used for the detection of IL-2 using 4-Fluoro-3-nitrophenyl grafted gold electrode based platform.
• It has been used for the production of tumor-specific cytotoxic T lymphocytes.
• It has been used for the proliferation of T lymphocytes.

Acciones bioquímicas o fisiológicas

IL-2/IL-2R (interleukin 2/IL-2 receptor) signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK (natural killer) cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. It is associated with recurrent oral ulcer and asthma and Mycoplasma pneumoniae infection in children. Absence of IL2 in mice causes fatal lymphoproliferative inflammatory disease (IL-2 deficiency syndrome). IL-2 signaling involves three pathways: phosphoinositol 3-kinase (PI3-K)/AKT, Ras-MAPK (mitogen activated protein kinase), and JAK-STAT (Janus kinase - signal transducers and activators of transcription) pathways.

Secuencia

MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFAQSII STLT

Forma física

Lyophilized with no additives.

Reconstitución

Reconstitute the contents of the vial using 100 mM acetic acid to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers and stored at 2-8 °C for up to 1 week. For extended storage, freeze in working aliquots. It may be advisable to centrifuge the vial prior to reconstitution.

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H319,H335
• Consejos de prudencia: P261 - P264 - P271 - P280 - P304 + P340 + P312 - P305 + P351 + P338
• Clasificaciones de peligro: Eye Irrit. 2 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1