SRP0237

PI3 kinase (p120g) Active human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

• Sinónimos: PIK3CG, Phosphoinositide-3-kinase, catalytic, gamma, p120-PI3K
• Código UNSPSC: 12352200
• NACRES: NA.32

Propiedades

• origen biológico: human
• recombinante: expressed in baculovirus infected insect cells
• Ensayo: ≥90% (SDS-PAGE)
• Formulario: aqueous solution
• actividad específica: ≥1.4 pmol/min-μg
• mol peso: 131 kDa
• envase: pkg of 20 μg
• concentración: >0.02 mg/mL
• Nº de acceso NCBI: AF327656
• Nº de acceso UniProt: P48736
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... PIK3CG(5294)

Descripción

Descripción general

Human p120γ ( GenBank Accession No. AF327656), full length with N-terminal His tag, MW = 131 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Aplicación

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Definición de unidad

One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

Forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Nota de preparación

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.