SRP0172

Jak2 Jh1Jh2 Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• Sinónimos: JAK2, JTK10, Janus kinase 2, Just another kinase 2, Tyrosine-protein kinase JAK2
• Código UNSPSC: 12352200
• NACRES: NA.32

Propiedades

• origen biológico: human
• recombinante: expressed in baculovirus infected insect cells
• Ensayo: ≥70% (SDS-PAGE)
• Formulario: aqueous solution
• mol peso: 99 kDa
• envase: pkg of 10 μg
• concentración: >0.02 mg/mL
• Nº de acceso NCBI: NM_004972
• Nº de acceso UniProt: O60674
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... JAK2(3717)

Descripción

Descripción general

Human Jak2 (GenBank Accession No. NM_004972), containing the catalytic and the pseudo kinase domain (amino acids 532-1132) with N-terminal GST-His tag, MW = 99 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Aplicación

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling

Definición de unidad

One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30°C.

Forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Nota de preparación

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable