SRP0113

HDAC-11 human

recombinant, expressed in baculovirus infected insect cells, ≥10% (SDS-PAGE)

• Sinónimos: HD11, Histone deacetylase 11
• Código UNSPSC: 12352200
• NACRES: NA.77

Propiedades

• origen biológico: human
• recombinante: expressed in baculovirus infected insect cells
• Análisis: ≥10% (SDS-PAGE)
• formulario: aqueous solution
• mol peso: 66 kDa
• envase: pkg of 50 μg
• condiciones de almacenamiento: avoid repeated freeze/thaw cycles
• concentración: >0.02 mg/mL
• Nº de acceso NCBI: BC009676
• Nº de acceso UniProt: Q96DB2
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... HDAC11(79885)

Descripción

Descripción general

HDAC-11 (histone deacetylase 11) is a zinc-dependent HDAC. It belongs to the HDAC family of proteins and HDAC class IV. It is highly conserved among various species. The mRNA is present in the brain, skeletal muscle, kidney and testis. The gene is mapped to human chromosome 3p25.
Human HDAC11, full length, GenBank Accession No. BC009676, with N-terminal GST tag, MW = 66kDa, expressed in baculovirus expression system.

Aplicación

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Acciones bioquímicas o fisiológicas

HDAC-11 (histone deacetylase 11) is responsible for deacetylation of nuclear histone and nonhistone proteins, including transcription factors. HDAC-11 catalyzes the deacetylation of histone H4. It binds to CDT1 (DNA replication factor) and is present with S phase-associated replication origins. HDAC-11 is upregulated in various carcinomas. Absence of it results in cell death and suppresses metabolic activity in colon, prostate, breast and ovarian cancer cell lines.

Definición de unidad

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 50% glycerol.

Nota de preparación

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable