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SBR00047

Sigma-Aldrich

Rhodamine B labeled Ramoplanin

For fluorescent microbial imaging

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About This Item

Fórmula empírica (notación de Hill):
C146H184ClN23O46S2
Peso molecular:
3096.73
Código UNSPSC:
12352116
NACRES:
NA.76

Nivel de calidad

Formulario

solid

actividad específica

Antibiotic (bacterial staining)

temp. de almacenamiento

−20°C

Descripción general

Rhodamine B labeled Ramoplanin is a fluorescent derivative of Ramoplanin, with a similar mode of action as Ramoplanin. It allows fluorescent labeling of Gram-positive bacteria and their detection by common fluorescent instruments. Ramoplanin is a lipoglycodepsipeptide antibiotic that is produced by the fermentation of Actinoplanes species. Fluorescent antibiotics are obtained by a synthetic conjugation of an antibiotic to a fluorophore.

Aplicación

Fluorescent antibiotics can be used for many applications including:
  • Antimicrobial resistance research
  • Bacterial visualization and imaging
  • Parent antibiotic mode of action research and new antibiotic discovery
  • Toxicity studies
  • Diagnosis of bacterial infections and tracking their uptake in vivo

Acciones bioquímicas o fisiológicas

Ramoplanin blocks the bacterial cell wall biosynthesis by specifically binding to the first sugar of lipid I. N-Acetylmuramic acid thus interfering with peptidoglycan production.

Mode of Action: Is different from the d-Ala-d-Ala that are targeted by vancomycin and shows no cross-resistance with other glycopeptides.

Activity Spectrum: Effective against a broad-spectrum activity against Gram-positive pathogens both in vitro and in vivo including Enterococci, Staphylococci, Bacilli, Streptococci, Listeria monocytogenes, and Gram-positive anaerobes such as Clostridrium difficile. However, Rhodamine B labeled Ramoplanin presents a MIC decrease in the magnitude of two to five orders in bacterial activity in comparison to the parent Ramoplanin.

Características y beneficios

High quality antibiotic suitable for mulitple research applications

Nota de análisis

  • Fluorescent microscopy application: Rhodamine B labeled Ramoplanin has excitation/emission wavelength range at 550-560 nm/590-600 nm.
  • It is recommended to dissolve the Rhodamine B labeled Ramoplanin in DMSO to a concentration of 2 mg/mL.
  • The recommended working concentration in fluorescent microscopy imaging application is 10μM. The mentioned concentration was used for Bacillus subtills staining (see image).
  • Aliquots of the DMSO solution can be stored at −20 °C, protected from light for at least one month.

Otras notas

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Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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