SAB5801018

Alpaca Anti-Mouse IgM(µ chain) single domain Antibody

recombinant Alpaca(VHH) Nano antibody

• Sinónimos: Recombinant Alpaca single variable domain secondary Nanobody
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: alpaca
• recombinante: expressed in E. coli
• conjugado: unconjugated (6*his tag and one cys were added at the C terminal of the VHH)
• forma del anticuerpo: affinity purified immunoglobulin
• tipo de anticuerpo: secondary antibodies
• clon: recombinant monoclonal
• Formulario: lyophilized solid
• mol peso: 14 kDa
• reactividad de especies: Alpaca, mouse
• técnicas: ELISA: 1:5000-1:20000; western blot: 1:5000-1:20000
• tamaño: 0.1 mL
• color: colorless
• isotipo: VHH
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Alpaca Anti-Mouse IgM(µ chain) single domain Antibody is designed to specifically detect mouse IgM. It is a monovalent, single domain, recombinant antibody derived from the variable region of heavy chain of Alpaca pacous. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Mouse IgM(µ chain) single domain Antibody reacts with the µ chain of mouse IgM selectively but not with mouse, rabbit, human, cynomolgus, rat, goat IgG.

Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. IgM accounts for 5-10% of the immunoglobulin pool and is the predominant antibody in the primary immune response. Unlike IgG, IgM does not contain a hinge region but does contain an additional constant domain. The monomeric form IgM has a molecular weight of 180 KD. It is classically represented as a pentamer of the basic four chain structure held together by a J chain but can also exist in a hexameric form without the J chain and as a monomer on the surface of B-cells.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Especificidad

Fc region of mouse IgM (µ chain specific)
Isotype: VHH domain of alpaca IgG2b/2c

Inmunógeno

Recombinant Alpaca single variable domain secondary nano antibody

Envase

100 μg in Glass bottle

Forma física

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

Reconstitución

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

Almacenamiento y estabilidad

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C

Otras notas

No cross-reactivity with mouse, rabbit, human, cynomolgus, rat, goat IgG

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H317,H412
• Consejos de prudencia: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Clasificaciones de peligro: Aquatic Chronic 3 - Skin Sens. 1
• Código de clase de almacenamiento: 13 - Non Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable