A1949
Anti-Rabbit IgG (γ-chain specific)–Peroxidase antibody, Mouse monoclonal
clone RG-96, purified from hybridoma cell culture
Sinónimos:
Monoclonal Anti-Rabbit IgG (γ-chain specific)
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.46
Productos recomendados
origen biológico
mouse
Nivel de calidad
conjugado
peroxidase conjugate
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
secondary antibodies
clon
RG-96, monoclonal
Formulario
lyophilized powder
reactividad de especies
rabbit
no debe reaccionar con
goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken
envase
vial of 0.5 mL
técnicas
direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot: 1:160,000 using total cell extract of HeLa cells (5-10 μg/well)
isotipo
IgG1
temp. de almacenamiento
2-8°C
modificación del objetivo postraduccional
unmodified
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Descripción general
Anti-Rabbit IgG (γ-chain specific)-Peroxidase antibody, Mouse monoclonal is a monoclonal anti-rabbit IgG conjugated to horseradish peroxidase. Monoclonal antirabbit IgG is derived from the RG-96 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rabbit IgG.
Rabbit IgGs are often used as primary antibodies in several immunological assays. Thus, polyclonal anti-rabbit IgGs are usually used as secondary antibodies in such applications. However, polyclonal antibodies to rabbit IgGs can also cross-react with IgGs from other species, especially with human IgGs, and thereby lead to a lack of specificity in test results. The use of monoclonal anti-rabbit IgG that is specific for only rabbit IgG improves the accuracy of immunological assays and thus, can be useful for various research applications.
The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.
Inmunógeno
Rabbit IgG
Aplicación
Monoclonal Anti-Rabbit IgG (γ-chain specific)- Peroxidase has been used in
- various immunochemical assays like enzyme linked immunosorbent assay (ELISA)
- westernblotting
- immunohistology
- dot immunobinding assay
Forma física
Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.
Nota de preparación
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Skin Sens. 1
Código de clase de almacenamiento
13 - Non Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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