SAB4700041

Anti-CD3, low endotoxin antibody, Mouse monoclonal

clone OKT3, purified immunoglobulin, buffered aqueous solution

• Sinónimos: Anti-CD3 Antibody
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• conjugado: unconjugated
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: OKT3, monoclonal
• formulario: buffered aqueous solution
• reactividad de especies: human
• concentración: 1 mg/mL
• técnicas: flow cytometry: suitable; immunohistochemistry: suitable
• isotipo: IgG2a
• Nº de acceso NCBI: NM_000733.3
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CD3(916)

Descripción

Descripción general

CD3 (cluster of differentiation 3) molecule is encoded by the gene mapped to human chromosome 11q23.3. The encoded protein exists in three isoforms, CD3ε, CD3γ and CD3δ and each contains an N-terminal extracellular domain, a transmembrane segment and a cytoplasmic domain. CD3 is a 20kDa glycoprotein expressed on the surface of all human T lymphocytes.

The mouse monoclonal antibody OKT3 recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody, also known as Orthoclone OKT3 or Muromonab-CD3, has been extensively used as a drug for therapy of acute, glucocorticoid resistant rejection of allogenic renal, heart and liver transplants. It has also been investigated for use in treating T-cell acute lymphoblastic leukemia.

Aplicación

Anti-CD3, low endotoxin antibody, mouse monoclonal has been used to activate Jurkat cells and peripheral blood mononuclear cell (PBMC) to facilitate formation of T helper (Tfh) cells.

The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 1 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Acciones bioquímicas o fisiológicas

T-cell receptor- CD3 (cluster of differentiation 3) complex including CD3ε, CD3γ and CD3δ play a vital role in inducing early metabolic events that lead to T cell activation. Mutation in CD3ε has been observed in T-B+ NK+ severe combined immunodeficiency (SCID) patients.

Características y beneficios

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Forma física

Solution in azide free phosphate buffered saline, pH 7.4; 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/mg of the protein, as determined by the LAL test.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable