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Merck

SAB4200759

Sigma-Aldrich

Anti-Human IgG3 antibody, Mouse monoclonal

clone HP-6050, purified from hybridoma cell culture

Sinónimos:

Anti-Immunoglobulin G3

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.43

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified from hybridoma cell culture

tipo de anticuerpo

primary antibodies

clon

HP-6050, monoclonal

formulario

buffered aqueous solution

reactividad de especies

human

concentración

~1.0 mg/mL

técnicas

immunoprecipitation (IP): 1-2.5 μg/test using Human IgG3 myeloma
indirect ELISA: 0.15-0.3 μg/mL using 1 μg/ml Human IgG3 myeloma for coating

isotipo

IgG1

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Descripción general

Human IgG consist of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. They constitute approximately 65, 30, 5 and 4% of the total IgG, respectively. The abundance of the different IgG subclasses in the bloodstream varies with age, IgG1 and IgG3 reach normal adult levels by 5-7 years of age while IgG2 and IgG4 levels rise more slowly, reaching adult levels at about 10 years of age.

Inmunógeno

Human IgG3 myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads

Aplicación

Anti-Human IgG3 antibody, mouse monoclonal has been used in indirect ELISA and immunoprecipitation.

Acciones bioquímicas o fisiológicas

IgG1 and IgG3 are the only subclasses capable of adherence to mononuclear phagocytes and are recognized readily by the Fc receptors on various reticulo-endothelial . IgG3 deficiency has been associated with a history of recurrent infectious, leading to chronic lung disease.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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R Jefferis et al.
Immunology letters, 10(3-4), 223-252 (1985-01-01)
Seventy-four monoclonal antibodies (McAb) of putative specificity for human IgG (11), the IgG sub-classes (59) or Gm allotypes (4) have been evaluated for reactivity and specificity in eight laboratories employing different assay techniques or protocols. For the IgG, IgG3, IgG4
Management of the patient with IgG subclass deficiency and/or selective antibody deficiency.
H G Herrod
Annals of allergy, 70(1), 3-8 (1993-01-01)
V A Oxelius
The American journal of medicine, 76(3A), 7-18 (1984-03-30)
The isotypes of IgG, IgG1, IgG2, IgG3, and IgG4 were determined in immunoglobulin preparations and the effect on serum levels of treated patients. Serum IgG subclass deficiencies were recorded in different patient groups: (1) IgG2-IgG4 deficiency was associated with IgA
R Stevens et al.
Journal of clinical immunology, 3(1), 65-69 (1983-01-01)
Peripheral blood leukocytes from individuals immunized with tetanus toxoid can be stimulated by pokeweed mitogen to produce IgG anti-tetanus toxoid antibody (IgG-Tet) in vitro. Previous studies have shown that treatment of these cells with tetanus toxoid or anti-human IgG reagents
F W van der Meulen et al.
British journal of haematology, 46(1), 47-56 (1980-09-01)
The purpose of this study was to determine whether quantitative or qualitative factors are of major importance in the destruction of red cells sensitized with incomplete warm autoantibodies of subclass IgG1. To that end, the relative amount of igG1 antibody

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