SAB4200473

Anti-EpCAM ICD antibody, Mouse monoclonal

clone 4A7, purified from hybridoma cell culture

• Sinónimos: Monoclonal Anti-DIAR5, Monoclonal Anti-EGP-2, Monoclonal Anti-EGP314, Monoclonal Anti-EGP40, Monoclonal Anti-ESA, Monoclonal Anti-EpCAM ICD antibody produced in mouse, Monoclonal Anti-HNPCC8, Monoclonal Anti-KS1/4, Monoclonal Anti-KSA, Monoclonal Anti-M4S1, Monoclonal Anti-MIC18, Monoclonal Anti-MK-1, Monoclonal Anti-TACSTD1, Monoclonal Anti-TROP1, Monoclonal Anti-epithelial cell adhesion molecule
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• conjugado: unconjugated
• forma del anticuerpo: purified from hybridoma cell culture
• tipo de anticuerpo: primary antibodies
• clon: 4A7, monoclonal
• formulario: buffered aqueous solution
• mol peso: antigen ~40 kDa
• reactividad de especies: human, mouse
• concentración: ~1.0 mg/mL
• técnicas: indirect immunofluorescence: 5-10 μg/mL using human MCF7 cells.; western blot: 0.25-0.5 μg/mL using whole extracts of human SW480 cells.
• isotipo: IgG1
• Nº de acceso UniProt: P16422
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... EPCAM(4072) ; mouse ... Epcam(17075)

Descripción

Descripción general

Monoclonal Anti- EpCAM ICD (mouse IgG1 isotype) is derived from the hybridoma 4A7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the intracellular domain of human EpCAM, conjugated to KLH. Epithelial cell adhesion molecule (EpCAM) is an epithelial transmembrane glycoprotein, composed of an ectodomain, a transmembrane domain and a cytoplasmic domain. EpCAM is frequently expressed in human malignancies, normal stem and progenitor cells, and at lower levels, in normal epithelia.

Inmunógeno

synthetic peptide corresponding to the intracellular domain of human EpCAM, conjugated to KLH. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Aplicación

Monoclonal Anti-EpCAM ICD antibody has been used in

• immunoblotting
• immunoprecipitation
• immunofluorescence

Acciones bioquímicas o fisiológicas

Epithelial cell adhesion molecule′s (EpCAM) intracellular domain (ICD) fragment translocates to the nucleus after cleavage from EpCAM ectodomain and associates with other molecules to bind DNA resulting in cell proliferation and tumor formation.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable