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Merck

SAB4200369

Sigma-Aldrich

Monoclonal Anti-Nectin-2/PVRL2 antibody produced in mouse

clone R2.5254.2, purified from hybridoma cell culture

Sinónimos:

Anti-CD112, Anti-HVEB, Anti-PRR2, Anti-PVRL2, Anti-PVRR2, Anti-Poliovirus receptor-related 2 (herpesvirus entry mediator B)

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

conjugado

unconjugated

forma del anticuerpo

purified from hybridoma cell culture

tipo de anticuerpo

primary antibodies

clon

R2.5254.2, monoclonal

formulario

buffered aqueous solution

mol peso

antigen ~72 kDa

reactividad de especies

rat, human, monkey, mouse

concentración

~1.0 mg/mL

técnicas

flow cytometry: 5-10 μg/test using using 1x106 MDA-MB-231 cells
immunocytochemistry: 2.5-5 μg/mL using using MDA-MB-231 cells
indirect ELISA: suitable
western blot: suitable

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

Descripción general

Monoclonal Anti-Nectin-2/PVRL2 (mouse IgG1 isotype) is derived from the hybridoma R2.525.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with 3T3 cells expressing human nectin-2. Nectin-2 is found to be widely expressed in human tissues, including hematopoietic cells.
Nectin-2, also known as poliovirus receptor related protein 2 gene (PRR2), is encoded by the gene mapped to human chromosome 19q13.2. The encoded protein belongs to the nectin protein family and is characterized with an extracellular region with three immunoglobulin-like loops, a single transmembrane domain and a cytoplasmic domain.

Especificidad

The antibody has been reported to block cell adhesion and herpes virus entry.

Inmunógeno

3T3 cells expressing human Nectin-2.

Aplicación

Monoclonal Anti-Nectin-2/PVRL2 antibody produced in mouse has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunocytochemistry
  • flow cytometry

Acciones bioquímicas o fisiológicas

Nectins act as key elements of adherens junctions and facilitate their formation. Nectin-2 possesses anti-viral property against herpes simplex virus (HSV)-2 infection. In mice, loss of nectin-2 affects sperm production and male-specific infertility. Aberrations in the gene has been associated with the pathogenesis of peripheral T-cell lymphoma (PTCL). Serum levels of nectin-2 can be used as a diagnostic and prognostic marker for colorectal cancer (CRC).

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

Elevated Nectin-2 expression is involved in esophageal squamous cell carcinoma by promoting cell migration and invasion
Li M, et al.
Oncology Letters, 15(4) (2018)
Serum nectin-2 levels are diagnostic and prognostic in patients with colorectal carcinoma.
Karabulut M
Clinical And Translational Oncology, 18, 160-171 (2016)
Sequencing of 42kb of the APO E-C2 gene cluster reveals a new gene: PEREC1.
Dna Sequence, 9, 89-100 (1998)
PVRL2 is translocated to the TRA@ locus in t(14;19)(q11;q13)-positive peripheral T-cell lymphomas.
Almire C
Genes Chromosomes Cancer, 46, 1011-1018 (2007)
Viral entry mechanisms: cellular and viral mediators of herpes simplex virus entry.
Akhtar J and Shukla D
FEBS Journal, 276, 7228-7236 (2009)

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