SAB4200255

Anti-PP2A subunit B isoform B56-δ antibody, Mouse monoclonal

clone H5D12, purified from hybridoma cell culture

• Sinónimos: Anti-PP2A B subunit isoform PR61-δ, Anti-PP2A B subunit isoform R5-δ, Anti-PPP2R5D, Anti-Protein phosphatase 2, regulatory subunit B′,δ, Anti-Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, δ isoform
• Código UNSPSC: 12352203
• NACRES: NA.44

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified from hybridoma cell culture
• tipo de anticuerpo: primary antibodies
• clon: H5D12, monoclonal
• formulario: buffered aqueous solution
• mol peso: antigen ~72 kDa
• reactividad de especies: human, rat
• concentración: ~1.0 mg/mL
• técnicas: immunoprecipitation (IP): suitable; indirect immunofluorescence: suitable; western blot: 2.5-5.0 μg/mL using whole extracts of human U-2-OS or rat Rat2 cells.
• isotipo: IgG1
• Nº de acceso UniProt: Q14738
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PPP2R5D(5528)

Descripción

Descripción general

Monoclonal Anti-PP2A subunit B isoform B56-δ (mouse IgG1 isotype) is derived from the hybridoma H5D12 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein. PP2A subunit B isoform B56 d (PPP2R5D) belongs to the phosphatase PP2A regulatory subunit B family. PP2A subunit B isoform B56 d (PPP2R5D) encodes a δ isoform of the regulatory subunit B56 subfamily. It is a member of phosphatase PP2A regulatory subunit B family. The core enzyme is a dimer composed of a catalytic subunit (C) and a constant regulatory subunit (A), that associates with a regulatory subunit (B).

Especificidad

The antibody recognizes human and rat PP2A B subunit isoform B56-δ. The antibody does not cross-react with PP2A-B55, PP2A-B56-α, PP2A-B56-β, PP2A-B56-γ or PP2A-B56-ε.

Inmunógeno

fusion protein corresponding to the N-terminus of human PP2A B subunit isoform B56δ.

Aplicación

Monoclonal Anti-PP2A subunit B isoform B56-δ antibody produced in mouse has been used in immunoblotting.

Acciones bioquímicas o fisiológicas

PP2A is a serine/threonine phosphatase that downregulates the MAP kinase pathway and subsequently modulates mitogenic signaling. This phosphatase also mediates cellular invasion, localization of Sgo1 at centromeres, cell movement, Ras-1 activation, and chromosome segregation. PP2A protein complex consists of a dimeric core containing subunits A and C; which in turn associates with the regulatory subunit B. PP2A subunit B is involved in the degranulation of mast cells .

Protein phosphatase 2A (PP2A) is implicated in the negative control of cell growth and division.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable