07-1221

Anti-PP2A-B′ (B56) Antibody

from rabbit

• Sinónimos: PP2A phosphatase activator, PP2A, subunit B′, PP2A, subunit B′, PR53 isoform, Phosphotyrosyl phosphatase activator, protein phosphatase 2A activator, regulatory subunit 4, protein phosphatase 2A, regulatory subunit B′, protein phosphatase 2A, regulatory
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: rat, human, mouse, bovine
• técnicas: western blot: suitable
• isotipo: IgG
• Nº de acceso NCBI: NM_006243
• Nº de acceso UniProt: Q15257
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PTPA(5524)

Descripción

Descripción general

PP2A is a heterotrimeric type 2 Ser/Thr phosphatase, composed of a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C). The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits. There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B′/PR61, and B′′/PR72 . The prototypical B (or PR55) family of subunits is encoded by three genes, Balpha, Bbeta and Bgamma . The B′ (or B56) family consists of at least seven splice variants derived from five genes. RNA′s for the two members of the B′′ family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene. Primary structures as well as functions of B and B′ subunits are conserved from yeast to mammals. PTPA is a member of the B′ family that is required for activation of PP2A by an undefined mechanism. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid. It is also thought to be the principal downmodulator of Ser/Thr kinase cascades.

Especificidad

This antibody is specific to PP2A-B’ subunits.

Inmunógeno

Epitope: Internal

The immunogen used for the purified peptide conjugated to KLH corresponds to the sequence NH2-Val-Ser-Ser-Pro-His-Phe-Gln-Val-Ala-Glu- Arg-Ala-Leu-Tyr-COOH.

Aplicación

Detect PP2A-B’ (B56) using this Anti-PP2A-B’ (B56) Antibody validated for use in WB.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

Kinases & Phosphatases

Calidad

Routinely evaluated by Western Blot on HeLa lysates.

Western Blot Analysis: 1:500-1:1,500 dilution of this antibody detected PP2A-B′ (B56) in 10 μg of multiple cell lysates.

Descripción de destino

~56 kDa

Ligadura / enlace

Replaces: AB4088

Forma física

Format: Purified

Purified

Purified rabbit polyclonal IgG in PBS with 0.08% sodium azide.

Almacenamiento y estabilidad

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análisis

Control
HeLa Cell Lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable