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S8761

Sigma-Aldrich

Sodium Bicarbonate

Solution (7.5%), sterile-filtered, suitable for cell culture

Sinónimos:

aqueous sodium bicarbonate

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About This Item

Número de CAS:
144-55-8
Número MDL:
MFCD00003528
Código UNSPSC:
12161702
ID de la sustancia en PubChem:
24899815
NACRES:
NA.75

Nombre del producto

Sodium bicarbonate solution, solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture

esterilidad

sterile-filtered

Nivel de calidad

400

Línea del producto

BioReagent

Formulario

solution (7.5%)

técnicas

cell culture | mammalian: suitable

impurezas

endotoxin, tested

pH

7.8-8.2

temp. de almacenamiento

2-8°C

cadena SMILES

[Na+].OC([O-])=O

InChI

1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1

Clave InChI

UIIMBOGNXHQVGW-UHFFFAOYSA-M

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Descripción general

Sodium bicarbonate (NaHCo3) is a commonly used alkaline buffering agent(5) in laboratories. The addition of this buffer to the cell culture medium aids in maintaining the appropriate pH by using the carbon dioxide present in the incubator. In addition to its buffering properties, Sodium bicarbonate also acts as a source of sodium and aids in sodium gluconate synthesis during the fermentation process.

Aplicación

Sodium bicarbonate solution has been used:
  • as a component of Hanks′ buffered salt solution (HBSS) and recording medium for culturing of tissue explants from rats or mice
  • as a component of Dulbecco′s modified Eagle′s medium (DMEM) to culture mice tissue explants
  • as a component of Minimum Essential Media (MEM) to study the cytotoxicity against human colorectal adenocarcinoma cells and mammary gland adenocarcinoma
  • to prepare collagen mix droplets for 3D collagen sprouting assay
  • to prepare collagen matrix for rat epidermal keratinocyte (REK) organotypic culture

Nota de preparación

Prepared in cell culture grade water.

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Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
RNBM0107
RNBL6582
RNBL5422
RNBK9943
RNBL0150

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Developmental and comparative immunology, 92, 140-149 (2018-11-20)
Viral double-stranded (ds)RNA is a potent pathogen-associated molecular pattern (PAMP), capable of inducing a strong antiviral state within the cell, protecting the cell from virus infection. In mammals and fish, sensing extracellular dsRNA is mediated by cell-surface class A scavenger
Mette Flinck et al.
Cell cycle (Georgetown, Tex.), 17(9), 1056-1067 (2018-06-14)
Precise acid-base homeostasis is essential for maintaining normal cell proliferation and growth. Conversely, dysregulated acid-base homeostasis, with increased acid extrusion and marked extracellular acidification, is an enabling feature of solid tumors, yet the mechanisms through which intra- and extracellular pH
Anna E Engler et al.
Cell reports, 33(13), 108553-108553 (2020-12-31)
There is an increasing appreciation for the heterogeneity of myeloid lineages in the lung, but relatively little is known about populations specifically associated with the conducting airways. We use single-cell RNA sequencing, flow cytometry, and immunofluorescence to characterize myeloid cells
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Journal of cell science, 133(17) (2020-08-14)
Neutrophils rely on glycolysis for energy production. How mitochondria regulate neutrophil function is not fully understood. Here, we report that mitochondrial outer membrane protein Mitofusin 2 (MFN2) regulates neutrophil homeostasis and chemotaxis in vivo Mfn2-deficient neutrophils are released from the
Le P Ngo et al.
Nucleic acids research, 48(3), e13-e13 (2019-12-12)
Genotoxicity testing is critical for predicting adverse effects of pharmaceutical, industrial, and environmental chemicals. The alkaline comet assay is an established method for detecting DNA strand breaks, however, the assay does not detect potentially carcinogenic bulky adducts that can arise
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Protocolos

Cultrex® 3-D Culture Matrix™ Rat Collagen I Protocol

3-D Culture Matrix™ Collagen I may be used as a gel on which to grow cells or a media additive alone or in concert with other basement membrane components to study cellular growth and differentiation in three dimensions in vitro.

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