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S6402

Sigma-Aldrich

Streptavidin−Cy3 from Streptomyces avidinii

buffered aqueous solution

Sinónimos:

Streptavidin Cy3

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

conjugado

CY3 conjugate

Nivel de calidad

Formulario

buffered aqueous solution

Extensión del etiquetado

3-9 mol fluorochrome per mol protein

técnicas

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

Descripción general

Streptavidin is a homotetramer protein. One molecule of streptavidin binds four molecules of biotin by a non-covalent interaction that is essentially irreversible. Streptavidin has a high affinity for biotin and is extensively used as a labeling tool and for studies involving binding proteins.

Aplicación

Streptavidin−Cy3 from Streptomyces avidinii has been used:
  • in double immunofluorescence staining to detect biotinylated lectin
  • for insulin and C-peptide staining in immunofluorescence analysis
  • in double immunofluorescence to verify the presence of cells filled with biocytin
  • in conjunction with biotinylated antibodies in immunohistochemistry and immunocytochemistry
  • with the traditional rhodamine filter arrangements due to the spectral overlap

Características y beneficios

Spectral Characteristics of Cy3 Dye:
  • Absorbance Max 552 nm
  • Emission Max 565 nm
  • Molar Extinction Coefficient 1.3 x 10-5 M-1cm-1

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide

Información legal

Cy3 is a trademark of Cytiva

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Agnieszka E Gorska et al.
The American journal of pathology, 163(4), 1539-1549 (2003-09-26)
We have previously shown that expression of a dominant-negative type II transforming growth factor-beta receptor (DNIIR) in mammary epithelium under control of the MMTV promoter/enhancer causes alveolar hyperplasia and differentiation in virgin mice. Here we show that MMTV-DNIIR female mice
Ji-Myung Bae et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 33(6), 1126-1140 (2018-02-07)
The Sp7/Osterix transcription factor is essential for bone development. Mutations of the Sp7 gene in humans are associated with craniofacial anomalies and osteogenesis imperfecta. However, the role of Sp7 in embryonic tooth development remains unknown. Here we identified the functional
Caroline Menard et al.
Nature neuroscience, 20(12), 1752-1760 (2017-12-01)
Studies suggest that heightened peripheral inflammation contributes to the pathogenesis of major depressive disorder. We investigated the effect of chronic social defeat stress, a mouse model of depression, on blood-brain barrier (BBB) permeability and infiltration of peripheral immune signals. We
A Bosio et al.
Cell and tissue research, 292(2), 199-210 (1998-06-02)
The myelin of central and peripheral nervous system of UDP-galactose-ceramide galactosyltransferase deficient mice (cgt-/-) is completely depleted of its major lipid constituents, galactocerebrosides and sulfatides. The deficiency of these glycolipids affects the biophysical properties of the myelin sheath and causes
Nam-Hoon Kim et al.
Plant biotechnology journal, 16(11), 1904-1917 (2018-04-01)
Panax ginseng C. A. Meyer, reputed as the king of medicinal herbs, has slow growth, long generation time, low seed production and complicated genome structure that hamper its study. Here, we unveil the genomic architecture of tetraploid P. ginseng by de

Artículos

Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.

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