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PP0100

Sigma-Aldrich

Trypsin Profile IGD Kit

Sinónimos:

In gel digestion kit, Proteomics grade Trypsin, TPCK treated

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.56

uso

 kit sufficient for ≤100 applications

Nivel de calidad

200

temp. de almacenamiento

2-8°C

Descripción general

The Trypsin Profile IGD Kit enables fast, efficient, and complete in-gel tryptic digestion of up to 100 excised protein spots. Digested proteins are ready for MALDI-MS (matrix-assisted laser desorption/ionization-mass spectrometry) and require no additional preparation. Because the Trypsin Profile IGD Kit contains Proteomics Grade Trypsin, a higher sequence coverage and fewer ambiguous autolytic peaks are observed in MALDI spectra.
Trypsin has been chemically modified through reductive methylation of the ε-amino groups of lysine to reduce autolysis and minimize autolytic fragments. In addition, it has been TPCK (tosyl phenylalanyl chloromethyl ketone) treated to remove residual chymotrypsin activity and then further purified by affinity chromatography, yielding a highly purified trypsin suitable for proteomics work.

Aplicación

Trypsin Profile IGD Kit has been used for in-gel digestion for LC-MS (liquid chromatography-mass spectrometry)/MS and MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass spectrometry analysis.

Características y beneficios

  • Faster destaining than with alternative in-gel digest kits
  • Includes 100% of the reagents needed to destain, digest, and extract proteins/peptides of interest
  • Compatible with Coomassie, SYPRO® Orange, SYPRO Ruby, and ProteoSilver™ Stained gels
  • Resulting samples are ready for analysis by MALDI-MS or HPLC-MS

Información legal

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC
SYPRO is a registered trademark of Life Technologies

Solo componentes del kit

Referencia del producto
Descripción
  • Destaining Solution Reconstituted to 75

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

3 - Flammable liquids

Punto de inflamabilidad (°F)

35.6 °F - closed cup

Punto de inflamabilidad (°C)

2.0 °C - closed cup

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Caroline L Weldon et al.
Toxicon : official journal of the International Society on Toxinology, 55(2-3), 558-569 (2009-10-20)
The Puerto Rican Racer Alsophis portoricensis is known to use venom to subdue lizard prey, and extensive damage to specific lizard body tissues has been well documented. The toxicity and biochemistry of the venom, however, has not been explored extensively.
Identification of plant viruses using one-dimensional gel electrophoresis and peptide mass fingerprints.
Luo, H., et al.
J. Virol. Methods, 165(2), 297-301 (2010)
SILAM for quantitative proteomics of liver Akt1/PKBa after burn injury.
Lu XM, et al.
International Journal of Molecular Medicine, 29, 461-461 (2012)
Shuo Han et al.
Cell death & disease, 12(11), 1070-1070 (2021-11-12)
Uncontrolled overactivation of autophagy may lead to autophagic cell death, suppression of which is a pro-survival strategy for tumors. However, mechanisms involving key regulators in modulating autophagic cell death remain poorly defined. Here, we report a novel long noncoding RNA
X-M Lu et al.
International journal of burns and trauma, 3(1), 37-48 (2013-02-07)
Insulin resistance is a major effect of burn injury and insulin receptor substrate-1 (IRS-1) plays an important role in signal transduction. Here, we explored the integrity of IRS-1 in muscle after burn injury. A murine model of severe burn injury
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