PLED35

GenElute^™ Endotoxin-free Plasmid Midiprep Kit

sufficient for 35 purifications

• Sinónimos: GenElute^™ Endotoxin-free Plasmid Kits, endo free midiprep plasmid isolation kit, endotoxin free plasmid extraction kit, endotoxin free plasmid isolation kit, endotoxin free plasmid purification kit, midiprep plasmid isolation kit, plasmid extraction kit, plasmid purification kit, Gen Elute
• Código UNSPSC: 41105501
• NACRES: NA.52

Propiedades

• uso: sufficient for 35 purifications
• Nivel de calidad: 200
• características de los productos alternativos más sostenibles: Inherently Safer Chemistry for Accident Prevention; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• técnicas: DNA purification: suitable
• categoría alternativa más sostenible: Aligned,
• temp. de almacenamiento: 15-25°C

Descripción

Descripción general

The GenElute Endotoxin-free Plasmid Kit offers a simple, rapid, cost effective method for purifying plasmid DNA with ≤0.1 EU/μg DNA for high efficiency transfection. Endotoxins (also known as lipopolysaccharides or LPS) are often co-purified with plasmid DNA and significantly reduce transfection efficiencies in endotoxin-sensitive cell lines.

Recombinant E. coli is harvested from an overnight culture by centrifugation and subjected to a modified alkaline-SDS lysis procedure to produce a cleared lysate. Endotoxins are removed from the cleared lysate with simple extraction-phase separation steps. Plasmid DNA is further purified by adsorption onto silica in the presence of high salts. After a simple spin-wash step, the bound plasmid DNA is eluted in endotoxin-free water. Up to 250 μg of endotoxin-free plasmid DNA may be prepared from 5 to 40 mL of culture.

The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as transfection, restriction digestion, cloning, sequencing, and PCR amplification.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Aplicación

The kit has been used for purifying plasmid DNA, which has been used for transfection.

Características y beneficios

• High transfection efficiency and yield
• Purify up to 250 μg endotoxin-free plasmid DNA (≤0.1 EU/μg DNA)
• Fast and simple allowing up to 12 preps in less than 2 hours

Información legal

GenElute is a trademark of Sigma-Aldrich Co. LLC

Información de seguridad

• Pictogramas: GHS08,GHS05,GHS07
• Palabra de señalización: Danger
• Frases de peligro: H290,H302 + H332,H315,H319,H334,H336
• Consejos de prudencia: P234 - P261 - P264 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Clasificaciones de peligro: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órganos de actuación: Central nervous system
• Código de clase de almacenamiento: 8A - Combustible corrosive hazardous materials
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable