P8279

Protocatechuate 3,4-Dioxygenase from Pseudomonas sp.

lyophilized powder, ≥3 units/mg solid

• Número de CAS: 9029-47-4
• Comisión internacional de enzimas: 1.13.11.3 (BRENDA, IUBMB)
• Número MDL: MFCD00132132
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• origen biológico: bacterial (Pseudomonas spp.)
• formulario: lyophilized powder
• actividad específica: ≥3 units/mg solid
• mol peso: ~700 kDa
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Protocatechuate 3,4-Dioxygenase belongs to the non-heme iron family of enzymes. The active site of the enzyme contains Fe³⁺.

Aplicación

Protocatechuate 3,4-Dioxygenase(PCD), from Pseudomonas sp., is used for the enzymatic determination of choline esterase when coupled with phydroxybenzoate hydroxylase. It is used to improve organic fluorophore-stability in single-molecule experiments and is used to study the metabolism of protocatechuate in Rhizobiaceae.

The enzyme has been used to create an oxygen scavenging system along with protocatechuate (PCA) and Trolox. The enzyme employs a nonheme iron center that catalyzes the conversion of PCA and molecular oxygen into β-carboxy-cis,cis-muconic acid, while the antioxidant Trolox suppresses slow blinking and photobleaching of cyanine dyes. It has been used in the preparation of imaging buffer along with DMB-BSA (dynein motility buffer-BSA), ATP and protocatechuate in single molecule motility assay.

Acciones bioquímicas o fisiológicas

Protocatechuate 3,4-Dioxygenase catalyzes the degradation of 3,4-dihydroxybenzoate (protocatechuate) into β-carboxy-cis,cis-muconate.

Propiedades físicas

Structure : Protein with nonheme iron
Inhibitors : Ag⁺, Hg^++, PCMB
Optimum pH : 9.0
Optimum temperature : 60−65°C
pH Stability : pH 7.0−9.0 (25°C, 72hr)
Thermal stability : below 50°C (pH 6.0, 1hr)

Definición de unidad

One unit will oxidize 1.0 μmole of protocatechuate to 3-carboxy-cis,cis-muconate per min at pH 7.5 at 37 °C.

Forma física

Supplied as lyophilized powder.

Nota de análisis

Protein determined by biuret.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)