345386

Glucose Oxidase, Aspergillus niger, Recombinant

Catalyzes the oxidation of β-D-glucose to D-glucono-ω-lactone and hydrogen peroxide. Also catalyzes the conversion of D-glucono-ω-lactone to gluconic acid.

• Sinónimos: Glucose Oxidase, Aspergillus niger, Recombinant
• Número de CAS: 9001-37-0
• Comisión internacional de enzimas: 1.1.3.4 (BRENDA, IUBMB)
• Número MDL: MFCD00131182
• Código UNSPSC: 12352202
• NACRES: NA.54

Propiedades

• Nivel de calidad: 100
• formulario: lyophilized solid
• actividad específica: ≥250 units/mg solid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• color: yellow-brown
• solubilidad: 0.1 M phosphate buffer, pH 7.0: 5 mg/mL
• actividad extraña: Amylase ≤0.1%; catalase ≤20 U/mg (dry weight); sucrase ≤0.1%
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• InChI: 1S/C6H12O6/c7-1-2-3(8)4(9)5(10)6(11)12-2/h2-11H,1H2/t2-,3-,4+,5-,6-/m1/s1
• Clave InChI: WQZGKKKJIJFFOK-VFUOTHLCSA-N

Descripción

Descripción general

Note: 1 KU = 1000 units.

Recombinant, Aspergillus niger glucose oxidase. Catalyzes the oxidation of β-D-glucose to D-glucono-ω-lactone and hydrogen peroxide. Also catalyzes the conversion of D-glucono-ω-lactone to gluconic acid. Each molecule of enzyme contains 2 molecules of FAD, which are essential for enzyme activity. Optimal pH = 6.5 with >95% maximal activity at pH 6.0-7.0. Useful for enzymatic assay of glucose. May also be conjugated to antibodies or antigens for use in ELISA procedures.

Recombinant, Aspergillus niger glucose oxidase. Catalyzes the oxidation of β-D-glucose to D-glucono-ω-lactone and hydrogen peroxide. Also catalyzes the conversion of D-glucono-ω-lactone to gluconic acid. Useful for enzymatic assay of glucose concentration.

Advertencia

Toxicity: Standard Handling (A)

Definición de unidad

One unit is defined as the amount of enzyme that will oxidize 1.0 mmol of β-D-glucose per minute at 25°C, pH 7.0.

Reconstitución

Following reconstitution, aliquot and refrigerate (4°C). Stock solutions are stable for up to 3 months at 4°C.

Otras notas

Heinz, F. and Beushausen, T.W. 1981. J. Clin. Chem. Clin. Biochem. 19, 977.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable