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P7181

Sigma-Aldrich

Polyethylene glycol solution

Hybri-Max™, 50 % (w/v), average mol wt 1,450, 0.2 μm filtered, BioReagent, suitable for hybridoma

Sinónimos:

PEG Solution, Polyethylene Glycol

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About This Item

Número MDL:
MFCD00081839
Código UNSPSC:
12161902
ID de la sustancia en PubChem:
24898829
NACRES:
NA.75

grado

Hybri-Max™

Nivel de calidad

200

esterilidad

0.2 μm filtered

Línea del producto

BioReagent

formulario

solution

mol peso

average mol wt 1,450

concentración

50 % (w/v)

técnicas

cell culture | hybridoma: suitable

impurezas

endotoxin, tested

temp. de almacenamiento

2-8°C

cadena SMILES

C(CO)O

InChI

1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2

Clave InChI

LYCAIKOWRPUZTN-UHFFFAOYSA-N

Categorías relacionadas

Descripción general

Polyethylene glycol (PEG) is a polymer of ethylene oxide and water, obtained upon condensation.

Aplicación

Polyethylene glycol solution has been used in the hybridization of the following:
  • irradiated camel fibroblast cells with A23 hamster cells
  • mouse splenocytes with Sp2/0-Ag 14 mouse myeloma cells
  • rabbit splenocytes and cell line 240E-1

Recommended for use in a normal fusion protocol requiring 50% PEG.

PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.

Acciones bioquímicas o fisiológicas

Polyethylene glycol (PEG) is used in detergents, emulsifying agents, soaps, metal costing process, pharmaceutical preparations and cosmetics. PEG is commonly used as a lubricant. It′s application is also observed in chromatographic stationary phases. PEG prevents crystal formation in emulsions.

Envase

Packaged in sealed ampules under nitrogen.

Otras notas

Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) in DPBS without calcium.

Reconstitución

Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.

Información legal

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

212.0 °F - closed cup

Punto de inflamabilidad (°C)

100 °C - closed cup

Equipo de protección personal

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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