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P3775

Sigma-Aldrich

Anti-Protein A antibody produced in rabbit

fractionated antiserum, lyophilized powder

Sinónimos:

Anti-Protein A

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

origen biológico

rabbit

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

fractionated antiserum

tipo de anticuerpo

primary antibodies

clon

polyclonal

Formulario

lyophilized powder

envase

vial of 2 mL lyophilized antiserum

técnicas

indirect ELISA: 1:100,000

temp. de almacenamiento

2-8°C

modificación del objetivo postraduccional

unmodified

Descripción general

Protein A is a 42 kDa bacterial protein. It is isolated from the cell wall of Staphylococcus aureus.
The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. To ensure specificity the fractionated antiserum is adsorbed using solid phase techniques, if necessary. Rabbit Anti-Protein A is lyophilized from 0.01 M phosphate buffered saline, pH 7.2, to which no preservatives have been added. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion versus anti-rabbit IgG results in a single arc of precipitation and versus anti-rabbit whole serum results in multiple arcs of precipitation.

Inmunógeno

Protein A from Staphylococcus aureus

Aplicación

Anti-Protein A antibody produced in rabbit has been used in immunoblotting.
Protein A may be used as in immunoblotting with a minimum dilution of 1:50,000.

Acciones bioquímicas o fisiológicas

Protein A has an ability to bind Fc region of immunoglobulins from several species. It is used as a “universal tracer” and it facilitate the segregation of bound and free ligand. Protein A also acts as affinity matrices for antibody (IgG) purification.

Forma física

Lyophilized from 0.01M phosphate buffered saline, pH 7.2

Reconstitución

Reconstitute with 2 ml deionized water.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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Human IgG subclasses
Antibody Fc, 159-177 (2014)
Smriti Kala et al.
PloS one, 7(10), e46864-e46864 (2012-10-12)
Most mitochondrial mRNAs in trypanosomatid parasites require uridine insertion/deletion RNA editing, a process mediated by guide RNA (gRNA) and catalyzed by multi-protein complexes called editosomes. The six oligonucleotide/oligosaccharide binding (OB)-fold proteins (KREPA1-A6), are a part of the common core of
Jitendra Thakur et al.
Eukaryotic cell, 10(10), 1295-1305 (2011-05-17)
A fungus-specific outer kinetochore complex, the Dam1 complex, is essential in Saccharomyces cerevisiae, nonessential in fission yeast, and absent from metazoans. The reason for the reductive evolution of the functionality of this complex remains unknown. Both Candida albicans and Schizosaccharomyces
IMMUNOASSAY CONFIGURATIONS
Immunoassay (1996)
Cell cycle-dependent phosphorylation and ubiquitination of a G protein alpha subunit
Torres MP, et al.
The Journal of Biological Chemistry, 286(23), 20208-20216 (2011)

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