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Merck

P3101

Sigma-Aldrich

Protein L from Peptostreptococcus magnus

>95% (SDS-PAGE), recombinant, expressed in E. coli, essentially salt-free, lyophilized powder

Sinónimos:

Protein L

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

recombinante

expressed in E. coli

Nivel de calidad

conjugado

unconjugated

ligand

human IgG (binding affinity 2 × 109 - 3 × 109 M-1)

Análisis

>95% (SDS-PAGE)

formulario

essentially salt-free, lyophilized powder

mol peso

35.8 kDa

técnicas

indirect ELISA: 1:100,000

temp. de almacenamiento

2-8°C

Descripción general

Protein L is an immunoglobulin light chain-binding protein. It is expressed by a few anaerobic bacterial species, such as Peptostreptococcus magnus.
Protein L protein binds immunoglobulin (Ig) light chains in a wide range of species including human, mouse, rat, pig, hamster, and chicken, but has low affinity for Igs from guinea pig, and rabbit. This bacterial protein interacts with Ig light chains without blocking the antigen binding site of the Igs . Thus, protein L can be used for the detection of IgG, IgM, IgA, IgE and IgD containing κ light chains. It may also be used to detect Fab and scFv fragments containing κ light chains. Protein L does not bind to bovine, sheep or goat Igs. Hence, when bound to a solid support (sepharose) , protein L may be used to directly purify monoclonal antibodies from media supplemented with BSA or FCS, and also to directly purify human or mouse antibodies from goat, bovine or sheep proteins. rProtein L is a recombinant form of the native protein and contains two Ig binding protein L fragments.

Aplicación

Protein L from Peptostreptococcus magnus is suitable for use in indirect ELISA (1:100,000). This product may also be used for immunohistochemistry in rat kidney sections .

Acciones bioquímicas o fisiológicas

Protein L from Peptostreptococcus magnus binds immunoglobulins (Ig) primarily through kappa light chain interactions without interfering with the antigen binding sites of Ig′s. This means that Protein L binds to a wider range of Ig classes and subclasses from a variety of species than any other commercially available Ig binding protein. It contains four Ig binding domains (B-domains). It may be used for the purification of IgG, IgM, IgA, IgE and IgD containing κ light chains and for the purification of Fab and scFv fragments containing κ light chains. It may be conjugated to a solid support for affinity purification or conjugated to marker molecules for use in detection. Protein L does not bind to bovine, sheep or goat immunoglobulins. For this reason it may be used for the purification of monoclonal antibodies from media supplemented with bovine serum albumin or fetal calf serum. It can also be used to purify human or mouse antibodies from goat, bovine or sheep proteins. A comprehensive list of binding properties is found in the product data sheet.

Reconstitución

Reconstitute with 5 mL PBS, pH 7.4 containing 15 mM sodium azide to yield a 1 mg/ml solution of Protein L. Other buffers may also be used for reconstitution. After reconstitution, the solution containing sodium azide may be stored at 2-8 °C for a maximum of 1 month.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct
Hoffert, J., D., et al.
American Journal of Physiology: Renal Physiology, 292(2), F691-F700 (2007)
Jia Deng et al.
Canadian journal of physiology and pharmacology, 95(2), 122-128 (2016-12-20)
Baicalin has been reported to attenuate lung edema in the process of lung injury. However, the effect of baicalin on alveolar fluid clearance (AFC) and epithelial sodium channel (ENaC) expression has not been tested. Sprague-Dawley rats were anesthetized and intratracheally
Protein L from Peptostreptococcus magnus binds to the kappa light chain variable domain.
Nilson BH, et al.
Test, 267(4), 2234-2239 (1992)
Complex between Peptostreptococcus magnus protein L and a human antibody reveals structural convergence in the interaction modes of Fab binding proteins
Graille M, et al.
Structure, 9(8), 679-687 (2001)
B H Nilson et al.
Journal of immunological methods, 164(1), 33-40 (1993-08-26)
Protein L from the bacterial species Peptostreptococcus magnus binds specifically to the variable domain of Ig light chains, without interfering with the antigen-binding site. In this work a genetically engineered fragment of protein L, including four of the repeated Ig-binding

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