P302-05

Porcine Pulmonary Artery Endothelial Cells: PPAEC (Cryovial)

• Código UNSPSC: 41106514
• NACRES: NA.81

Propiedades

• origen biológico: Porcine pulmonary artery (Normal healthy)
• Nivel de calidad: 100
• Formulario: solid
• envase: pkg of 500,000 cells
• fabricante / nombre comercial: Cell Applications, Inc
• modo de crecimiento: Adherent
• cariotipo: 2n = 38
• morfología: Endothelial
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: diabetes; cardiovascular diseases
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Descripción general

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PPAEC have been used in a pioneer study of endothelial cell motility, where it was determined that asymmetric dimethylarginine (ADMA), an inhibitor of nitric oxide production and a cardiovascular risk factor, decreased PKG-mediated phosphorylation of VASP, leading to downregulation of Rac1 and induction of stress fibers and focal adhesions via RhoA/ROCK dependent mechanism, leading to decreased cell motility, and increase in endothelial permeability, thus linking vascular repair and function with NO production (Wojciak-Stothard, 2007, 2009). PPAEC were also used to assess endothelial adhesion to decellularized porcine arteries in order to develop grafts for human vascular repair (Xiong, 2013).

Origen línea celular

Artery

Aplicación

cardiovascular function, screening of drug candidates, angiogenesis, vasodilator function, transport of deoxygenated bloood from heart to lungs

Componentes

Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO

Nota de preparación

• 1st passage, >500,000 cells in Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO
• Can be cultured at least 16 doublings

Rutina de subcultivo

Please refer to the PPAEC Culture Protocol.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable