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M5524

Sigma-Aldrich

Murashige and Skoog Basal Salt Mixture (MS)

powder, suitable for plant cell culture

Sinónimos:

MS Basal Salts, MS0 Basal Salts, MSO Basal Salts

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.72

Formulario

powder

Nivel de calidad

100

técnicas

cell culture | plant: suitable

aplicaciones

agriculture

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

Categorías relacionadas

Descripción general

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and Kinetin (cytokinin/cell division promoter).

Aplicación

Murashige and Skoog Basal Salt Mixture (MS) has been used to support the growth and regeneration of transformed tobacco cells. It has also been used as a growth medium for germination of Arabidopsis thaliana (Col-0) seeds.

Variante de la fórmula

With the macro- and micronutrients as described by Murashige and Skoog (1962).

Media Formulation

Cantidad

Formulated to contain 4.3 grams of powder per liter of medium.

Nota de preparación

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Salt mixture (M5524) contains the macronutrients and micronutrients of the original classic formulation. It can be combined with M&S vitamins or Gamborg′s vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Otras notas

Storage
Powdered media are extremely hygroscopic and must be protected from atmospheric moisture. If possible the entire contents of each package should be used immediately after opening.

Store dry medium in a desiccator at 0-5 °C.

Deterioration of powdered medium may be recognized by: 1) color change; 2) granulation, clumping, or particulate matter throughout the powder; 3) insolubility; 4) pH change; or 5) inability to promote growth when properly used.

Media Preparation

Producto relacionado

Referencia del producto
Descripción
Precios

08168

Timestrip Plus™ 8 °C

Pictogramas

Flame over circleExclamation mark
GHS03,GHS07

Palabra de señalización

Warning

Frases de peligro

H272,H319

Clasificaciones de peligro

Eye Irrit. 2 - Ox. Sol. 3

Código de clase de almacenamiento

5.1B - Oxidizing hazardous materials

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Listados normativos

Los listados normativos se proporcionan para los productos químicos principalmente. Para los productos no químicos sólo se puede proporcionar información limitada. Si no hay ninguna entrada, significa que ninguno de los componentes está en la lista. Es obligación del usuario garantizar el uso seguro y legal del producto.

EU REACH SVHC Candidate List

CAS No.

EU REACH Annex XVII (Restriction List)

CAS No.

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Certificados de análisis (COA)

Lot/Batch Number
SLCH8257
SLCF5871
SLCF0742
SLCC6355
SLCC5900

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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Pyae Phyo et al.
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Classical Plant Media Formulations

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

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