O7264

Anti-Osteopontin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Sinónimos: Anti-Bone Sialoprotein 1, Anti-Early T Lymphocyte Activation Protein 1 (ETA1), Anti-Secreted Phosphoprotein 1, Anti-Uropontin
• Número MDL: MFCD02263865
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• Formulario: buffered aqueous solution
• mol peso: antigen ~50 kDa
• reactividad de especies: human
• técnicas: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5-5 μg/mL using human kidney; western blot: 0.5-1 μg/mL using whole extract of human embryonal kidney 293T cells and a chemiluminescent detection reagent
• Nº de acceso UniProt: P10451
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... SPP1(6696)

Descripción

Descripción general

Osteopontin (OPN), also termed as bone sialoprotein 1, urinary stone protein, early t lymphocyte activation protein 1 (ETA1), and uropontin, is a phosphorylated acidic arginine-glycine-aspartate (RGD)-containing glycoprotein that binds integrin and CD44 receptors. OPN is the most abundant product of osteoblast and kidney cells, it is also expressed in lung, liver, bladder, pancreas, breast, vascular smooth muscle cells and macrophages.

Secreted phosphoprotein 1 (SPP1) or osteopontin is a secreted cytokine. This phosphorylated acidic glycoprotein is expressed in various cells and tissues. It is synthesized by osteoblasts. The gene encoding SPP1 is localized on human chromosome 4q22.1.

Inmunógeno

synthetic peptide corresponding to amino acid residues 249-267 of human osteopontin, con­jugated to KLH.

Aplicación

Anti-Osteopontin antibody produced in rabbit has been used in immunohistochemistry and immunoblotting.

Acciones bioquímicas o fisiológicas

Osteopenia (OPN), functions like chemokine and cytokine. It is implicated in a number of physiologic and pathologic events including angiogenesis, apoptosis, inflammation, wound healing and tumor metastasis. Osteopontin expression is upregulated in cancer of breast, prostate, ovarian, lung, brain tumor and hepatocellular carcinoma.

Secreted phosphoprotein 1 (SPP1) or osteopontin modulates inflammatory responses and glucose metabolism. It is a chemotactic factor and has a role in immune cell recruitment. The protein is also associated with cell growth, migration and differentiation. SPP1 attaches osteoclasts to the bone matrix. It is also involved in atherosclerotic process and bone mineralization. It is a marker of many cancers.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)