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NPT01

Sigma-Aldrich

NeuroPorter™ Transfection Kit

Lipid formulation for nucleic acid transfections in neuronal and glial cells

Sinónimos:

NeuroPorter Transfection, Transfection Kit

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.25

grado

for molecular biology

Nivel de calidad

200

Formulario

dried film

uso

 kit sufficient for 75-200 transfections

disponibilidad

available only in USA, Canada and EU

técnicas

transfection: suitable

temp. de almacenamiento

2-8°C

Categorías relacionadas

Descripción general

Neuroporter™ Transfection Reagent is a unique formulation of a proprietary cationic lipid optimized for delivery of DNA into primary neurons, glial cells, and cultured neuronal cell lines with high efficiency and low toxicity. The Neuroporter™ Transfection Kit was designed for difficult-to-transfect primary neurons, addressing past problems such as poor cell viability, low transfection efficiency and neuro-degeneration.

Aplicación

Suitable for transient and stable transfection of nucleic acids into primary neurons and cultured neuronal cell lines. Use approximately 15-120 μl Neuroporter™ Transfection Reagent and 6-8 μg DNA (in provided unique DNA Dilution buffer when required) per 6 cm cell culture plate. The following cells have been successfully transfected using the Neuroporter™ Transfection Kit:

  • C6 glioma (human)
  • Cortical neurons (rat primary)
  • Dorsal Root Ganglion (DRG) cells (rat)
  • NT2 neurons(human precursor cells)
  • NT neurons (human differentiated cells)
  • Subventricular Zone (SVZ) cells (mouse)
  • White matter cells (mouse)

Características y beneficios


  • Optimized for primary neurons, glial cells, and cultured neural cell lines
  • Very low toxicity with no neuro-degeneration or dendrite withdrawal
  • Efficient DNA delivery primary neurons, glial cells, and cultured neural cell lines
  • Fast and easy to use compared to other methods
  • Compatible with both serum and serum-free transfection protocols

Componentes

1 vial Neuroporter™ Transfection Reagent, dried lipid film (T2823)
1.5 mL Hydration Buffer H9036
7.5 mL DNA Diluent D1941

Precaución

Do not freeze.

Principio

A stable, non-covalent complex is formed when the Neuroporter™ Transfection Reagent is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.

Información legal

NeuroPorter is a trademark of Gene Therapy Systems, Inc.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
SLCJ6956
SLCH2000
SLCC1660
SLBZ1116
SLBW7289

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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Nikhil G Thaker et al.
Journal of neuroscience methods, 185(2), 204-212 (2009-09-29)
A major challenge for the treatment of cancers, such as glioblastoma multiforme (GBM), has been resistance to radiation and cancer chemotherapeutics. Short interfering RNA (siRNA) based screening may facilitate the identification of genes and pathways essential for cancer cell survival
Ling Luo et al.
eLife, 2, e00324-e00324 (2013-06-26)
Optimal phototransduction requires separation of the avascular photoreceptor layer from the adjacent vascularized inner retina and choroid. Breakdown of peri-photoreceptor vascular demarcation leads to retinal angiomatous proliferation or choroidal neovascularization, two variants of vascular invasion of the photoreceptor layer in
Adan Aguirre et al.
Nature, 467(7313), 323-327 (2010-09-17)
Specialized cellular microenvironments, or 'niches', modulate stem cell properties, including cell number, self-renewal and fate decisions. In the adult brain, niches that maintain a source of neural stem cells (NSCs) and neural progenitor cells (NPCs) are the subventricular zone (SVZ)
Beata Jablonska et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(42), 14775-14793 (2012-10-19)
Diffuse white matter injury (DWMI) caused by hypoxia is associated with permanent neurodevelopmental disabilities in preterm infants. The cellular and molecular mechanisms producing DWMI are poorly defined. Using a mouse model of neonatal hypoxia, we demonstrate a biphasic effect on
Simone Di Giovanni et al.
The Journal of biological chemistry, 280(3), 2084-2091 (2004-11-04)
Following spinal cord injury, there are numerous changes in gene expression that appear to contribute to either neurodegeneration or reparative processes. We utilized high density oligonucleotide microarrays to examine temporal gene profile changes after spinal cord injury in rats with
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Artículos

Introduction to Cell Transfection

Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

Contenido relacionado

Transfection Reagent Selection Guide

Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.

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