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NA2010

Sigma-Aldrich

GenElute™ Blood Genomic DNA Kit

sufficient for 70 purifications

Sinónimos:

Blood Genomic DNA, Gen Elute

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About This Item

Código UNSPSC:
41105501
NACRES:
NA.55

uso

sufficient for 70 purifications

Nivel de calidad

200

técnicas

DNA purification: suitable

temp. de almacenamiento

15-25°C
room temp

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Categorías relacionadas

Descripción general

The GenElute Blood Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from fresh or aged (older than 24 hours) whole blood. The kit combines the advantages of silica binding with a microspin format, and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Aplicación

GenElute™ Blood Genomic DNA Kit has been used:
  • to extract DNA from samples of large granules (LG) and smaller granules (SG) obtained from vasectomized rabbits
  • to isolate DNA from the blood samples 
  • to extract whole blood DNA from the left blood cells

The purified genomic DNA is ready for downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • sequencing reactions
  • cloning

Características y beneficios

  • Starting material: Up to 200 μl of fresh or aged blood
  • Expected yield: Up to 10 μg
  • Elution volume: 400 μl
  • Time required: <40 min
  • A260/A280 ratio: 1.6 - 1.9
  • Compatible with many anticoagulants, including EDTA, Heparin, and Sodium Citrate
  • Typical yields 4-10 μg from 200 μL of whole blood
  • Pure genomic DNA with a A260/A280 ratio between 1.6 and 1.9
  • Isolate DNA up to 50 kb in length

Principio

The starting material is lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. A Prewash Solution is provided to help remove contaminants that are associated with aged (older than 24 hours) whole blood samples. After washing to remove contaminants, the DNA is eluted in 200 mL of a Tris-EDTA solution.

The expected yields of genomic DNA will vary depending on the amount and nature of the starting material used (for example, 4 to 10 μg of RNase A-treated DNA can be isolated from 200 μl of fresh whole blood in less than one hour). DNA purified with this kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Otras notas

For additional information, please see www.sigma-aldrich.com/genomicdna.

Información legal

GenElute is a trademark of Sigma-Aldrich Co. LLC

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • C2112Column Preparation SolutionSDS
  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biologySDS
  • R6148RNase A solutionSDS

Opcional

Referencia del producto
Descripción
Precios

Producto relacionado

Referencia del producto
Descripción
Precios

P2308

Proteinasa K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology

E1510

Ethidium bromide solution, BioReagent, for molecular biology, 10 mg/mL in H2O

A9539

Agarosa, BioReagent, for molecular biology, low EEO

L3287

Escort™ IV Transfection Reagent, Lipid reagent for transient and stable transfection of mammalian and insect cells.

T6400

Tris-Borate-EDTA buffer, 5× Concentrate

D9780

Lambda DNA Hind III Digest, for DNA electrophoresis

R6148

RNase A solution

NA2020

GenElute™ Blood Genomic DNA Kit, sufficient for 350 purifications

C2112

Column Preparation Solution

93221-001

RNAstable®, Tube Kit, for room temp preservation of RNA

T0956

Sigma Universal Transfection Reagent, Polymer reagent for transfecting common mammalian cell lines

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

10 - Combustible liquids

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Certificados de análisis (COA)

Lot/Batch Number
SLCL5155
SLCJ7933
SLCF4479
SLCF4478
SLCC6972

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Extraction & Amplification of Whole Blood Using WGA-Protocol

Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield. This protocol is a simple method to isolate DNA from fresh or aged whole blood products. Once the DNA is isolated, it can be amplified using the GenomePlex® Whole Genome Amplification protocol.

Blood Card - Extraction & Amplification WGA Protocol

Blood cards provide the convenience of archiving small volumes of blood. However, many times genomic DNA from these samples is limited, This protocol provides a simple and convenient method to extract genomic DNA from a blood card. Once the DNA has been extracted, it can then be amplified using the amplification protocol

GenElute™ Blood Genomic DNA Kit Protocol
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