Saltar al contenido
Merck
Todas las fotos(1)

Documentos

NA1111

Sigma-Aldrich

GenElute™ Gel Extraction Kit

sufficient for 70 purifications

Sinónimos:

DNA gel extraction kit, DNA gel isolation kit, DNA gel purification kit, PCR gel clean up kit, PCR gel isolation kit, gel isolation kit, gel purification kit, Rapid purification of linear DNA

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

Código UNSPSC:
41105501
NACRES:
NA.52

uso

sufficient for 70 purifications

Nivel de calidad

200

técnicas

DNA purification: suitable

temp. de almacenamiento

15-25°C

Categorías relacionadas

Descripción general

The GenElute Gel Extraction Kit is designed for the rapid purification of linear and plasmid DNA fragments from standard or low-melting agarose gels. This kit can also be used to purify DNA from polyacrylamide gels. Typical recovery of DNA is up to 80%. Each column can bind up to 10 μg of DNA from up to a 3.5 g agarose slice.

Aplicación

GenElute™ Gel Extraction Kit has been used for the purification of DNA fragments from agarose gels.
The isolated DNA is suitable for a variety of downstream applications, such as sequencing, PCR, restriction digestion, cloning and labeling.

Características y beneficios

  • Bind up to 10 μg of DNA
  • Recoveries up to 80%
  • Up to 3.5 g can be processed per column
  • Compatible with both standard and low-melting agarose in TAE or TBE buffer

Principio

The GenElute Gel Extraction Kit combines silica-binding technology with the convenience of a spin or vacuum column format. DNA fragments of interest are extracted by solubilizing slices of an agarose gel. The Gel Solubilization Solution dissolves an agarose gel slice from gels run in TBE or TAE buffer. The extracted DNA fragments selectively adsorb onto the silica membrane in the presence of the Gel Solubilization Solution. Contaminants are removed by a simple spin or vacuum wash. Finally, the bound DNA is eluted in Tris buffer.
The GenElute Gel Extraction Kit combines silica-binding technology with the convenience of a spin or vacuum column format. DNA fragments of interest are extracted from slices of an agarose gel and are bound to a silica membrane. Contaminants are removed by a simple spin or vacuum wash. The bound DNA is then eluted.

The purified DNA is suitable for a variety of downstream applications, such as automated DNA sequencing, PCR, restriction digestion, cloning, and labeling.

Información legal

GenElute is a trademark of Sigma-Aldrich Co. LLC

Opcional

Referencia del producto
Descripción
Precios

Pictogramas

CorrosionExclamation mark
GHS05,GHS07

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1C - STOT SE 3

Órganos de actuación

Respiratory system

Riesgos supl.

EUH032

Código de clase de almacenamiento

8A - Combustible, corrosive hazardous materials

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Attempts to Construct an Enlarged pUC19 via Insertion of HindIII-digested Coliphage ? DNA
Muhamed Amirie
Journal of Experimental Microbiology and Immunology, 18, 96-101 (2014)
K Michael Lee et al.
Yeast (Chichester, England), 22(6), 431-440 (2005-04-26)
The Saccharomyces cerevisiae ADH2 promoter (P(ADH2)) is repressed several hundred-fold in the presence of glucose; transcription is initiated once the glucose in the medium is exhausted. The promoter can thus be utilized for effective regulation of recombinant gene expression in
Prolactin inhibits a major tumor-suppressive function of wild type BRCA1.
Chen KH and Walker Am
Cancer Letters, 375(2), 293-302 (2016)
Vernon K Ward et al.
Proceedings of the National Academy of Sciences of the United States of America, 104(26), 11050-11055 (2007-06-22)
Noroviruses are the major cause of nonbacterial gastroenteritis in humans. These viruses have remained refractory to detailed molecular studies because of the lack of a reverse genetics system coupled to a permissive cell line for targeted genetic manipulation. There is
Charles S Cockell et al.
The ISME journal, 5(10), 1671-1682 (2011-05-20)
An epilithic microbial community was launched into low Earth orbit, and exposed to conditions in outer space for 548 days on the European Space Agency EXPOSE-E facility outside the International Space Station. The natural phototroph biofilm was augmented with akinetes
Ver todos los artículos relacionados

Protocolos

GenElute™ Gel Extraction procedure

The GenElute Gel Extraction kit is designed for the rapid purification of 50 bp to 10 kb linear DNA fragments and plasmids from standard or low-melting agarose gels.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico