MAK478

α-Amylase Activity Assay Kit

sufficient for 100 colorimetric tests

• Sinónimos: Assay Kit for Amylase Activity
• Código UNSPSC: 12352204
• NACRES: NA.84

Propiedades

• Nivel de calidad: 100
• aplicaciones: agriculture; food and beverages
• método de detección: colorimetric
• enfermedades relevantes: endocrinological disorders; gastrointestinal diseases; genitourinary disorders
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Amylase belongs to the family of glycoside hydrolase enzymes that break down starch into glucose molecules by acting on α-1,4- glycosidic bonds. The α-amylases cleave at random locations on the starch chain, ultimately yielding maltotriose and maltose, glucose and "limit dextrin" from amylose and amylopectin. In mammals, α-amylase is a major digestive enzyme. Increased enzyme levels in humans are associated with salivary trauma, mumps due to inflammation of the salivary glands, pancreatitis, and renal failure.

Características y beneficios

• The linear detection range of the kit is 0.3 to 50 U/L α-amylase.

Idoneidad

The kit is suitable for the determination of α-amylase activity in blood, saliva, urine, grains, and other agricultural samples.

Principio

Simple, direct, and automation-ready procedures for measuring amylase activity are very desirable. The α-Amylase Activity Assay Kit method involves two steps. First, α -amylase in the sample hydrolyses starch and the product is rapidly converted to glucose by α-glucosidase and hydrogen peroxide by glucose oxidase. Next, the hydrogen peroxide concentration is determined with a colorimetric reagent at 585 nm.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2