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MAK189

Sigma-Aldrich

A-KG Dehydrogenase Activity Assay Kit

Sufficient for 100 Colorimetric tests

Sinónimos:

α-KG Dehydrogenase Activity Assay Kit, α-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit, AKGDH Assay Kit, Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit, OGDC Activity Assay Kit, Oxoglutarate Dehydrogenase Assay Kit

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

método de detección

colorimetric

enfermedades relevantes

endocrinological disorders, diabetes; orthopedic diseases; neurological disorders; rheumatological diseases; cancer; cardiovascular diseases

temp. de almacenamiento

−20°C

Información sobre el gen

human ... OGDH(4967)
mouse ... OGDH(18293)
rat ... OGDH(360975)

Descripción general

α-Ketoglutarate Dehydrogenase (α-KGDH, EC 1.2.4.2) is a highly regulated Krebs cycle enzyme, forms the E1 subunit of the mitochondrial 2-oxoglutarate dehydrogenase complex. It catalyzes the conversion of α-ketoglutarate to succinyl-CoA and produces NADH directly providing electrons for the respiratory chain. Decreased α-KGDH complex activity has been associated with oxidative stress and neurodegenerative disorders such as Alzheimer′s and Parkinson′s diseases.

Aplicación

α-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit has been used to measure the enzyme activity of α-ketoglutarate dehydrogenase in samples.

Idoneidad

Suitable for the detection of α-ketoglutarate dehydrogenase activity in samples such as tissue, cells, and purified mitochondria.

Principio

The α-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit provides a simple procedure for measuring α-ketoglutarate dehydrogenase activity in a variety of tissues, cells, and isolated mitochondria. α-KGDH activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of α-ketoglutarate dehydrogenase is the amount of enzyme that generates 1.0 μmole of NADH per minute at pH 7.5 at 37 °C.

Solo componentes del kit

Referencia del producto
Descripción
  • KGDH Assay Buffer
  • KGDH Substrate
  • KGDH Developer
  • NADH Standard
  • KGDH Positive Control

Pictogramas

Health hazardCorrosion
GHS08,GHS05

Palabra de señalización

Danger

Frases de peligro

H314,H334

Clasificaciones de peligro

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Código de clase de almacenamiento

8A - Combustible corrosive hazardous materials

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Poldip2 is an oxygen-sensitive protein that controls PDH and αKGDH lipoylation and activation to support metabolic adaptation in hypoxia and cancer.
Paredes F, et al.
Proceedings of the National Academy of Sciences of the USA, 115(38), 201720693-201720693 (2018)
Sirtuin lipoamidase activity is conserved in bacteria as a regulator of metabolic enzyme complexes.
Rowland E A, et al.
mBio, 8(5), e01096-e01017 (2017)
Alpha-ketoglutarate dehydrogenase: a target and generator of oxidative stress.
Tretter L and Vera A
Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 360(1464), 2335-2345 (2005)
Alexandre Buffet et al.
The Journal of clinical endocrinology and metabolism, 106(2), 459-471 (2020-11-13)
Pheochromocytomas and paragangliomas (PPGLs) are neuroendocrine tumors in which altered central metabolism appears to be a major driver of tumorigenesis, and many PPGL genes encode proteins involved in the tricarboxylic acid (TCA) cycle. While about 40% of PPGL cases carry
Elizabeth A Rowland et al.
mBio, 8(5) (2017-09-14)
Lipoic acid is an essential metabolic cofactor added as a posttranslational modification on several multimeric enzyme complexes. These protein complexes, evolutionarily conserved from bacteria to humans, are core regulators of cellular metabolism. While the multistep enzymatic process of adding lipoyl
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