MAK161

Multidrug Resistance Assay Kit

(Fluorometric MDR Assay)

• Sinónimos: MDR Assay
• Código UNSPSC: 12161503
• NACRES: NA.84

Propiedades

• uso: (Fluorometric MDR Assay)
• aplicaciones: pharmaceutical
• método de detección: fluorometric
• enfermedades relevantes: cancer
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... ABCB1(5243) , ABCC2(1244) ; mouse ... ABCC2(12780) ; rat ... abcc2(25303)

Descripción

Descripción general

Acquired resistance to chemotherapy drugs, multidrug resistance or MDR, is a major contributor to treatment failure for many types of cancers. MDR is typically associated with the increased expression of two ATP-dependent drug efflux pumps, P-Glycoprotein (P-gp or MDR1) and the Multidrug Resistance-Associated Protein (MRP1). These pumps actively expel chemotherapeutic agents, typically hydrophobic amphipathic natural products, from the cytoplasm to exterior of the cell.

This kit is utilizes a hydrophobic fluorescent dye molecule to assess MDR activity in cells. This dye rapidly penetrates cell membranes and becomes trapped resulting in an increase in fluorescence intensity (λ_ex = 490/λ_em = 525 nm). In cells expressing MDR transporters, the dye is rapidly extruded by the transporters, resulting in decreased fluorescence intensity.

Características y beneficios

Compatible with high-throughput handling systems.

Idoneidad

This kit is suitable for the screening of MDR pump inhibitors or for identifying cell lines with high MDR activity.

Principio

This kit is utilizes a hydrophobic fluorescent dye molecule to assess MDR activity in cells. This dye rapidly penetrates cell membranes and becomes trapped resulting in an increase in fluorescence intensity (λ_Ex = 490/λ_Em = 525 nm). In cells expressing MDR transporters, the dye is rapidly extruded by the transporters, resulting in decreased fluorescence intensity.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids