MAK107

LCAT Activity Assay Kit

Supplied by Roar Biomedical, Inc.

• Sinónimos: Lecithin:cholesterol acyltransferase activity assay kit
• Número CE: 200-659-6
• Código UNSPSC: 12161503
• NACRES: NA.84

Propiedades

• uso: sufficient for 100 fluorometric tests
• aplicaciones: pharmaceutical
• método de detección: fluorometric
• enfermedades relevantes: cardiovascular diseases
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... LCAT(3931)

Descripción

Descripción general

The plasma protein lecithin:cholesterol acyltransferase (LCAT) catalyzes the transfer of an acyl group from the sn2 position of phosphatidylcholine to the 3-hydroxyl group of cholesterol resulting in the formation cholesteryl ester. This enzymatic activity occurs on the surface of high density lipoprotein (HDL). The cholesteryl esters formed by LCAT may be packed into the core of HDL.

Aplicación

Applications for this method include high-throughput screening, mechanism of action studies and structure-activity relationship (SAR) work.

LCAT Activity Assay Kit has been used for measuring lecithin-cholesterol acyl transferase activity in plasma samples.

Características y beneficios

Compatible with high-throughput handling systems.

Idoneidad

Suitable for measuring phospholipase activity of LCAT in plasma or serum.

Principio

The LCAT Activity Assay Kit is a fluorometric assay useful for measuring phospholipase activity of (λ_Ex=390 nm/λ_Em=470 nm) LCAT. The assay may be validated by inhibition of LCAT with iodoacetate or another spectrally benign (not Ellman′s reagent) LCAT inhibitor.

Nota de análisis

Learn more about Roar Assay Kits for Studies in Lipoprotein Metabolism.

Solo componentes del kit

• Substrate Reagent .1 mL
• READ Reagent 30 mL
• LCAT Assay Buffer 20 mL

Información de seguridad

• Pictogramas: GHS02,GHS06,GHS08
• Palabra de señalización: Danger
• Frases de peligro: H225,H301 + H311 + H331,H370
• Consejos de prudencia: P210 - P233 - P280 - P301 + P310 - P303 + P361 + P353 - P304 + P340 + P311
• Clasificaciones de peligro: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
• Código de clase de almacenamiento: 3 - Flammable liquids
• Punto de inflamabilidad (°F): 49.5 °F
• Punto de inflamabilidad (°C): 9.7 °C