M6065

Monoclonal Anti-Microphthalmia antibody produced in mouse

clone C5, purified immunoglobulin

• Sinónimos: Anti-Mi
• Número MDL: MFCD00802742
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: C5, monoclonal
• formulario: buffered aqueous solution
• mol peso: antigen 52-56 kDa
• reactividad de especies: mouse, rat, human
• concentración: 0.5-1.0 mg/mL
• técnicas: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable using 2 μg/mg protein lysate; western blot: 1:500 using 10 μg of Mouse brain lysates
• isotipo: IgG1
• Nº de acceso UniProt: O75030
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... MITF(4286) ; mouse ... Mitf(17342) ; rat ... Mitf(25094)

Descripción

Descripción general

Microphthalmia (Mi in mouse or MITF in human) is a basic helix-loop-helix-leucine zipper (BHLH–ZIP) transcription factor involved in the differentiation, development and survival of melanocytes and cells of the retinal pigment epithelium, i.e. cells responsible for hair, skin, and eye color. It activates the expression of the melanocyte specific genes tyrosinase and TRP1 (tyrosinase-related protein 1) by binding as a homo- or heterodimer to a symmetrical DNA sequence (E box) (5′-CATGTG-3′) located within the M box found in their promoters. Microphthalmia also appears to be involved in the differentiation of mast cells, osteoclasts, basophils and natural killer cells.
Microphthalmia is expressed in a limited number of cell types including heart, mast cells, osteoclast precursors, and melanocytes. There are a number of different isoforms of microphthalmia resulting from alternative splicing and alternative promotors. These isoforms differ at their amino-termini and in their expression patterns.

Mouse monoclonal clone C5 anti-Microphthalmia antibody recognizes serine phosphorylated and non-phosphorylated melanocytic isoforms of microphthalmia from human, mouse or rat.

Inmunógeno

N-terminal fragment of human microphthalmia protein.

Aplicación

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Mouse monoclonal clone C5 anti-Microphthalmia antibody is used to tag serine phosphorylated and non-phosphorylated melanocytic isoforms of microphthalmia for detection and quantitation by techniques such as immunoblotting (doublet of 52-56 kDa), immunoprecipitation, immunohistochemistry on formalin-fixed paraffin embedded or frozen tissue sections, and gel shift. It is used as a probe to determine the roles of microphthalmia in the differentiation, development and survival of melanocytes and cells of the retinal pigment epithelium.

Forma física

Solution in phosphate buffered saline containing 0.08% sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable