Saltar al contenido
Merck

11718096001

Roche

DAB Substrate

dark brown, /black precipitate; Visually evaluated

Sinónimos:

3,3′-diaminobenzidine tetrahydrochloride, DAB

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

Código UNSPSC:
12352204

fabricante / nombre comercial

Roche

Nivel de calidad

color

dark brown, /black precipitate; Visually evaluated

temp. de almacenamiento

−20°C

Descripción general

The metal enhanced DAB Substrate utilizes cobalt chloride and nickel chloride in a special formulation to produce a dark brown/black precipitate in the presence of horseradish peroxidase (HRP).
3, 3′ diaminobenzidine tetrahydrochloride (DAB) is a carcinogen that should be used safely. It is one of the major sensitive substrates for HRP (horseradish peroxidase). DAB is brown or amber in color and becomes insoluble and precipitates over the antigen-antibody site due to the presence of hydrogen peroxide and peroxidase.

Aplicación

  • DAB Substrate is a color sustrate for peroxidases and is used in a variety of applications:Immunohistocytochemistry
  • In situ hybridization
  • Western blot
  • Dot blot
DAB substrate has been used in immunofluorescence and western blot.

Envase

1 set containing 2 components.

Nota de preparación

Working solution:
  1. Determine the volume of substrate required for the development of peroxidase.
  2. Remove the DAB/metal concentrate (10x) from -15 to -25 °C storage.
    Note: Do not bring to 15 to 25 °C.
  3. Dilute the DAB/metal concentrate (10x) with the peroxide buffer to a 1x working solution. For example, if 5 ml of substrate are required, dilute 500 μl of the DAB/metal concentrate (10x) with 4.5 ml of the Peroxide buffer.
  4. Mix well.
  5. Add the 1x working solution to the tissue or blot until desired substrate development.
    Note: The 1x solution is stable over many hours.
    For best results, store the working solution at 2 to 8 °C when not using.

Storage conditions (working solution): 2 to 8 °C
For best results, store the working solution at 2 to 8 °C when not using.

Otras notas

For life science research only. Not for use in diagnostic procedures.

Solo componentes del kit

Referencia del producto
Descripción

  • DAB/metal concentrate, 25 ml 10x concentrated

  • Peroxide Buffer, 250 ml

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 4 - Aquatic Chronic 2 - Carc. 1A - Carc. 1B - Flam. Liq. 2 Oral - Repr. 1B - Skin Sens. 1 Inhalation - STOT RE 2 - STOT SE 1

Código de clase de almacenamiento

3 - Flammable liquids

Clase de riesgo para el agua (WGK)

WGK 3


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

João Francisco Botelho et al.
Proceedings. Biological sciences, 281(1788), 20140765-20140765 (2014-06-27)
The zygodactyl orientation of toes (digits II and III pointing forwards, digits I and IV pointing backwards) evolved independently in different extant bird taxa. To understand the origin of this trait in modern birds, we investigated the development of the
Pathobiology of Human Disease: A Dynamic Encyclopedia of Disease Mechanisms (2014)
Adrenergic Receptor Protocols (2000)
The developmental origin of zygodactyl feet and its possible loss in the evolution of Passeriformes.
Botelho J F, et al.
Proc. Royal Soc. Lond. B., 281(1788), 20140765-20140765 (2014)
Principles and Reactions of Protein Extraction, Purification, and Characterization (2005)

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico