M4812

Anti-Myosin Va (LF-18) antibody produced in rabbit

~0.4 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinónimos: Anti-GS1, Anti-MYH12, Anti-MYO5, Anti-MYR12
• Número MDL: MFCD03455613
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• formulario: buffered aqueous solution
• mol peso: antigen 190 kDa
• reactividad de especies: chicken, rat
• validación mejorada: independent; Learn more about Antibody Enhanced Validation
• concentración: ~0.4 mg/mL
• técnicas: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using rat and chicken cerebellum sections; microarray: suitable; western blot: 1:1,000 using a rat brain extract
• Nº de acceso UniProt: Q02440
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... MYO5A(4644) ; mouse ... Myo5a(17918) ; rat ... Myo5a(25017)

Descripción

Descripción general

Anti-myosin Va (LF-18) recognizes myosin Va (190kDa). Myosin 5a is encoded by the gene mapped to human chromosome 15q21.2. The encoded protein is a member of the unconventional myosin family. It is a two-headed motor containing two identical heavy chains and multiple light chains. Myosin Va is widely expressed, but at high levels in the brain, testes and melanocytes.

Inmunógeno

synthetic peptide located near the C-terminus of chicken myosin Va (amino acids 1782-1799 with N-terminally added lysine) conjugated to KLH. This sequence is identical in human, mouse and rat.

Aplicación

Anti-Myosin Va (LF-18) antibody produced in rabbit has been used in:

• Western blot.
• In situ proximity ligation assay (PLA).
• Glutathione S-transferase (GST)-spinophilin co-sedimentation assay.

Acciones bioquímicas o fisiológicas

Myosin 5a is an actin-based molecular motor responsible for transport of secretory granules in various cell systems, including insulin granule. Additionally, it also mediates the transport of cargo such as cell-surface receptors, pigment and organelles like endoplasmic reticulum to the plasma membrane. The encoded protein facilitates pigmentation and synaptic activity in the central nervous system. Genetic variations in the gene are associated with the development of immunological and neurological diseases, including Griscelli syndrome type 1 and Elejalde syndrome.

Forma física

Solution in 0.01 M phosphate buffered saline containing 1% BSA and 15 mM sodium azide.

Almacenamiento y estabilidad

For continuous use, store at 2-8°C for up to one month. For prolonged storage, freeze in working aliquots at -20°C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Otras notas

In order to obtain best results in different techniques and preparations we recommend determining optimal working dilutions by titration test.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable